Anti-KCNT1抗体[N3/26] (ab94578)

概述

  • 产品名称
    Anti-KCNT1抗体[N3/26]
    参阅全部 KCNT1 一抗
  • 描述
    小鼠单克隆抗体[N3/26] to KCNT1
  • 宿主
    Mouse
  • 经测试应用
    适用于: Flow Cyt, WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Fusion protein: QDEMNDHHQN TLSYVLINPP PDTRLEPNDI VYLIRSDPLA HVTSSSQSRK SSCSNKLSSC NPETRDETQL, corresponding to amino acids 1168-1237 of Rat Kcnt1 (NP_068625)

  • 阳性对照
    • Rat brain lysate
  • 常规说明

    The clone number has been updated from S3-26 to N3/26, both clone numbers name the same antibody clone.

     

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    N3/26
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab94578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 137 kDa.
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.

靶标

  • 功能
    Outwardly rectifying potassium channel subunit that may co-assemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro).
  • 组织特异性
    Highest expression in liver, brain and spinal cord. Lowest expression in skeletal muscle.
  • 序列相似性
    Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa4.1/KCNT1 sub-subfamily.
    Contains 1 RCK N-terminal domain.
  • 翻译后修饰
    Phosphorylated by protein kinase C. Phosphorylation of the C-terminal domain increases channel activity.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA100C15.2 antibody
    • EIEE14 antibody
    • ENFL5 antibody
    • KCa4.1 antibody
    • KCNT1 antibody
    • KCNT1_HUMAN antibody
    • Potassium channel subfamily T member 1 antibody
    • Potassium channel, sodium activated subfamily T, member 1 antibody
    • Potassium channel, subfamily T, member 1 antibody
    • Sequence like a calcium-activated K+ channel antibody
    • SLACK antibody
    • Slo2.2 antibody
    see all

图片

  • Overlay histogram showing SH-SY5Y cells stained with ab94578 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab94578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

文献

ab94578 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
10 µg
Treatment
Dox 24 h
Specification
HEK293
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Apr 03 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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