E coli-derived fusion protein (1), 293 cell extract (2), and Hela cell extract (3) as test antigen. Affinity-purified IgY dilution: 1:2000 (or 1:500 for cell extract), Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
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Chen M et al. Voltage-gated K(+) channels sensitive to stromatoxin-1 regulate myogenic and neurogenic contractions of rat urinary bladder smooth muscle. Am J Physiol Regul Integr Comp Physiol299:R177-84 (2010).
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