概述

  • 产品名称Anti-KCNQ2抗体
    参阅全部 KCNQ2 一抗
  • 描述
    兔多克隆抗体to KCNQ2
  • 特异性NB: Rabbit polyclonal to KCNQ2 (ab22897) was used in reducing but not in denaturing conditions in WB (i.e. not heated or boiled).
  • 经测试应用适用于: IHC-FoFr, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human KCNQ2.

    (Peptide available as ab25449.)

性能

应用

Our Abpromise guarantee covers the use of ab22897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 20592205
WB Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). NB: Rabbit polyclonal to KCNQ2 (ab22897) was used in reducing but not in denaturing conditions in WB (i.e. not heated or boiled).
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Probably important in the regulation of neuronal excitability. Associates with KCNQ3 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. KCNQ2/KCNQ3 current is blocked by linopirdine and XE991, and activated by the anticonvulsant retigabine. Muscarinic agonist oxotremorine-M strongly suppress KCNQ2/KCNQ3 current in cells in which cloned KCNQ2/KCNQ3 channels were coexpressed with M1 muscarinic receptors.
  • 组织特异性In adult and fetal brain. Highly expressed in areas containing neuronal cell bodies, low in spinal chord and corpus callosum. Isoform 2 is preferentially expressed in differentiated neurons. Isoform 6 is prominent in fetal brain, undifferentiated neuroblastoma cells and brain tumors.
  • 疾病相关Defects in KCNQ2 are the cause of benign familial neonatal seizures type 1 (BFNS1) [MIM:121200]. A disorder characterized by clusters of seizures occurring in the first days of life. Most patients have spontaneous remission by 12 months of age and show normal psychomotor development. Some rare cases manifest an atypical severe phenotype associated with epileptic encephalopathy and psychomotor retardation. The disorder is distinguished from benign familial infantile seizures by an earlier age at onset. In some patients, neonatal convulsions are followed later in life by myokymia, a benign condition characterized by spontaneous involuntary contractions of skeletal muscles fiber groups that can be observed as vermiform movement of the overlying skin. Electromyography typically shows continuous motor unit activity with spontaneous oligo- and multiplet-discharges of high intraburst frequency (myokymic discharges). Some patients may have isolated myokymia.
    Defects in KCNQ2 are the cause of epileptic encephalopathy early infantile type 7 (EIEE7) [MIM:613720]. EIEE7 is an autosomal dominant seizure disorder characterized by infantile onset of refractory seizures with resultant delayed neurologic development and persistent neurologic abnormalities.
  • 序列相似性Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.2/KCNQ2 sub-subfamily.
  • 结构域The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • 翻译后修饰In Xenopus oocytes KCNQ2/KCNQ3 heteromeric current can be increased by intracellular cyclic AMP, an effect that depends on phosphorylation of Ser-52 in the N-terminus region.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BFNC antibody
    • BFNS1 antibody
    • EBN 1 antibody
    • EBN antibody
    • EBN1 antibody
    • EIEE7 antibody
    • ENB 1 antibody
    • ENB1 antibody
    • HNSPC antibody
    • KCNA 11 antibody
    • KCNA11 antibody
    • KCNQ 2 antibody
    • Kcnq2 antibody
    • KCNQ2_HUMAN antibody
    • KQT like 2 antibody
    • KQT-like 2 antibody
    • KV7.2 antibody
    • KVEBN 1 antibody
    • KVEBN1 antibody
    • KvLQT 2 antibody
    • KvLQT2 antibody
    • Neuroblastoma specific potassium channel alpha subunit KvLQT2 antibody
    • Neuroblastoma specific potassium channel protein antibody
    • Neuroblastoma specific potassium channel subunit alpha antibody
    • Neuroblastoma specific potassium channel subunit alpha KvLQT2 antibody
    • Neuroblastoma-specific potassium channel subunit alpha KvLQT2 antibody
    • OTTHUMP00000031681 antibody
    • OTTHUMP00000031682 antibody
    • OTTHUMP00000031684 antibody
    • OTTHUMP00000031685 antibody
    • OTTHUMP00000031686 antibody
    • OTTHUMP00000031687 antibody
    • OTTHUMP00000031689 antibody
    • Potassium voltage gated channel KQT like protein 2 antibody
    • Potassium voltage gated channel KQT like subfamily member 2 antibody
    • Potassium voltage gated channel subfamily KQT member 2 antibody
    • Potassium voltage-gated channel subfamily KQT member 2 antibody
    • Voltage gated potassium channel subunit Kv7.2 antibody
    • Voltage-gated potassium channel subunit Kv7.2 antibody
    see all

Anti-KCNQ2 antibody 图像

  • All lanes : Anti-KCNQ2 antibody (ab22897) at 1 µg/ml

    Lane 1 : Rat whole brain lysate
    Lane 2 : Mouse whole brain lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    Anti-rabbit HRP at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 95 kDa
    Observed band size : 95 kDa

    This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

    Rabbit polyclonal to KCNQ2 (ab22897) was used in reducing conditions in WB but not in denaturing conditions (i.e. not heated).

  • Immunfluorescent detection of KCNQ2 (green colour) in differentiated murine neural precursors using Rabbit polyclonal to KCNQ2 (ab22897). KCNQ2 labeling is seen in the tips of neurite extensions (such as the cell in the top left corner), along axons, dendrites and possibily spines. ab22897 (3.5µg/ml) was incubated overnight at 4°C. Secondary antibody used is goat anti-rabbit Alexa fluor 488 (1h at RT ). The red colour is MAP2 detection (Secondary used is goat anti-mouse Alexa fluor 568; 1h at RT). NB:  The white centres are caused by high intensity to-pro-3 blue nuclear counterstain cross-colouring with the red and background green.

  • All lanes : Anti-KCNQ2 antibody (ab22897) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 95 kDa
    Observed band size : 95 kDa
    Additional bands at : 140 kDa,15 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab22897 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Abcam recommends loading unheated lysates. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

Anti-KCNQ2 antibody (ab22897)参考文献

This product has been referenced in:
  • Salzer I  et al. Phosphorylation regulates the sensitivity of voltage-gated Kv7.2 channels towards phosphatidylinositol-4,5-bisphosphate. J Physiol N/A:N/A (2016). Read more (PubMed: 27621207) »
  • Tomonoh Y  et al. The kick-in system: a novel rapid knock-in strategy. PLoS One 9:e88549 (2014). IHC (PFA fixed) ; Mouse . Read more (PubMed: 24586341) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (mouse spinal cord)
Specification mouse spinal cord
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 25 2015

Application Western blot
Sample Rat Tissue lysate - whole (hippocampus (lane B) and cortex (lane C))
Loading amount 30 µg
Specification hippocampus (lane B) and cortex (lane C)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Sophie Pezet

Verified customer

提交于 May 18 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Whole brain lysate - adult)
Loading amount 40 µg
Specification Whole brain lysate - adult
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

提交于 May 18 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (neurones differentiated from neural precursors)
Specification neurones differentiated from neural precursors
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

提交于 May 12 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"