概述

  • 产品名称Anti-KCNQ1抗体
    参阅全部 KCNQ1 一抗
  • 描述
    山羊多克隆抗体to KCNQ1
  • 特异性This antibody is expected to recognize both reported isoforms (NP_000209.2; NP_861463.1).
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-EQLTVPRRGPDEGS

    , corresponding to C terminal amino acids 662-676 of Human KCNQ1 (NP_000209.2).

  • 阳性对照
    • Human heart tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab106812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 75 kDa.

靶标

  • 功能Potassium channel that plays an important role in a number of tissues, including heart, inner ear, stomach and colon (By similarity) (PubMed:10646604). Associates with KCNE beta subunits that modulates current kinetics (By similarity) (PubMed:9312006, PubMed:9108097, PubMed:8900283, PubMed:10646604, PubMed:11101505, PubMed:19687231). Induces a voltage-dependent by rapidly activating and slowly deactivating potassium-selective outward current (By similarity) (PubMed:9312006, PubMed:9108097, PubMed:8900283, PubMed:10646604, PubMed:11101505). Promotes also a delayed voltage activated potassium current showing outward rectification characteristic (By similarity). During beta-adrenergic receptor stimulation participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks) (By similarity) (PubMed:9312006, PubMed:9108097, PubMed:8900283, PubMed:10646604, PubMed:11101505). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current (PubMed:10713961). When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions (PubMed:10646604). This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents (By similarity). During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion (By similarity). Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion (By similarity). When associated with KCNE2, forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (PubMed:11101505). When associated with KCNE4, inhibits voltage-gated potassium channel activity (PubMed:19687231). When associated with KCNE5, this complex only conducts current upon strong and continued depolarization (PubMed:12324418). Also forms a heterotetramer with KCNQ5; has a voltage-gated potassium channel activity (PubMed:24855057). Binds with phosphatidylinositol 4,5-bisphosphate (PubMed:25037568).
    Isoform 2: Non-functional alone but modulatory when coexpressed with the full-length isoform 1.
  • 组织特异性Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries.
  • 疾病相关Long QT syndrome 1
    Jervell and Lange-Nielsen syndrome 1
    Atrial fibrillation, familial, 3
    Short QT syndrome 2
    Diabetes mellitus, non-insulin-dependent
  • 序列相似性Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.1/KCNQ1 sub-subfamily.
  • 结构域The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The coiled-coil domain mediates tetramerization.
    The segment S6 is involved in the inhibition of voltage-gated potassium channel activity by KCNE4.
    The C-terminal assembly domain promotes self-interactiona; allows functional channel.
  • 翻译后修饰Phosphorylation at Ser-27 by PKA; increases delayed rectifier potassium channel activity of the KCNQ1-KCNE1 complex through a macromolecular complex that includes PKA, PP1, and the targeting protein AKAP9.
    Ubiquitinated by NEDD4L; promotes internalization (PubMed:22024150). The ubiquitinylated form is internalized through a clathrin-mediated endocytosis by interacting with AP2M1 and is recycled back to the cell membrane via RAB4A and RAB11A (PubMed:23529131).
    Deubiquitinated by USP2; counteracts the NEDD4L-specific down-regulation of I(Ks) and restores the membrane localization.
  • 细胞定位Cell membrane. Cytoplasmic vesicle membrane. Early endosome. Membrane raft. Endoplasmic reticulum. Basolateral cell membrane. Colocalized with KCNE3 at the plasma membrane (PubMed:10646604). Upon 17beta-oestradiol treatment, colocalizes with RAB5A at early endosome (PubMed:23529131). Heterotetramer with KCNQ5 is highly retained at the endoplasmic reticulum and is localized outside of lipid raft microdomains (PubMed:24855057). During the early stages of epithelial cell polarization induced by the calcium switch it removed from plasma membrane to the endoplasmic reticulum where it retained and it is redistributed to the basolateral cell surface in a PI3K-dependent manner at a later stage (PubMed:21228319).
  • Information by UniProt
  • 数据库链接
  • 形式There are 2 isoforms produced by alternative splicing. Isoform 2 also known as: TKvLQT1.
  • 别名
    • ATFB1 antibody
    • ATFB3 antibody
    • FLJ26167 antibody
    • IKs producing slow voltage-gated potassium channel subunit alpha antibody
    • IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1 antibody
    • Jervell and Lange-Nielsen syndrome 1 antibody
    • JLNS1 antibody
    • KCNA8 antibody
    • KCNA9 antibody
    • KCNQ1 antibody
    • KCNQ1_HUMAN antibody
    • kidney and cardiac voltage dependend K+ channel antibody
    • KQT-like 1 antibody
    • Kv1.9 antibody
    • Kv7.1 antibody
    • KVLQT1 antibody
    • long (electrocardiographic) QT syndrome, Ward-Romano syndrome 1 antibody
    • LQT antibody
    • LQT1 antibody
    • Potassium channel, voltage-gated, shaker-relatd subfamily, member 9 antibody
    • Potassium voltage-gated channel subfamily KQT member 1 antibody
    • potassium voltage-gated channel, KQT-like subfamily, member 1 antibody
    • RWS antibody
    • slow delayed rectifier channel subunit antibody
    • SQT2 antibody
    • Voltage-gated potassium channel subunit Kv7.1 antibody
    • WRS antibody
    see all

Anti-KCNQ1 antibody 图像

  • Anti-KCNQ1 antibody (ab106812) at 1 µg/ml + Human heart tissue lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 75 kDa

Anti-KCNQ1 antibody (ab106812)参考文献

ab106812 has not yet been referenced specifically in any publications.

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