Anti-KCNN4抗体(ab75956)

概述

  • 产品名称Anti-KCNN4抗体
    参阅全部 KCNN4 一抗
  • 描述
    兔多克隆抗体to KCNN4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human KCNN4.

    (Peptide available as ab87266.)

  • 阳性对照
    • This antibody gave a positive signal in the following human tissue lysates: Skin Placenta

性能

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应用

Our Abpromise guarantee covers the use of ab75956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
  • 组织特异性Widely expressed in non-excitable tissues.
  • 序列相似性Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.
  • 翻译后修饰Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hIKCa1 antibody
    • hKCa4 antibody
    • hSK4 antibody
    • IK1 antibody
    • IKCa1 antibody
    • Intermediate conductance calcium activated potassium channel protein 4 antibody
    • Intermediate conductance calcium-activated potassium channel protein 4 antibody
    • KCa3.1 antibody
    • KCa4 antibody
    • KCNN 4 antibody
    • Kcnn4 antibody
    • KCNN4_HUMAN antibody
    • Potassium intermediate/small conductance calcium activated channel, subfamily N, member 4 antibody
    • Putative Gardos channel antibody
    • SK4 antibody
    • SKCa 4 antibody
    • SKCa4 antibody
    see all

Anti-KCNN4 antibody 图像

  • All lanes : Anti-KCNN4 antibody (ab75956) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-KCNN4 antibody (ab75956)参考文献

ab75956 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience. Here is the homology between human and mouse for the following antibodies (based on the alignment of their immunogen sequences) ab55452: homology = 94% - crossreactivity very likely, but not tested ab57662: homolog...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - whole (Right Coronary Artery & Lung)
Loading amount 20 µg
Specification Right Coronary Artery & Lung
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-20% Tris Ready Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Aug 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"