Anti-KCNN3抗体(ab28631)

概述

  • 产品名称Anti-KCNN3抗体
    参阅全部 KCNN3 一抗
  • 描述
    兔多克隆抗体to KCNN3
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide derived from residues 647 - 696 of human KCNN3.

    .

  • 阳性对照
    • Daudi cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab28631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/62500.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 82 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.
  • 序列相似性Belongs to the potassium channel KCNN family. KCa2.3/KCNN3 subfamily.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hSK3 antibody
    • KCa2.3 antibody
    • Kcnn3 antibody
    • KCNN3_HUMAN antibody
    • Potassium calcium activated channel subfamily N member 3 antibody
    • Potassium channel calcium activated intermediate/small conductance subfamily N alpha member 3 antibody
    • Potassium channel, calcium activated, intermediate/small conductance, subfamily N, member 3 antibody
    • Potassium intermediate/small conductance calcium activated channel subfamily N member 3 antibody
    • SK3 antibody
    • SKCa 3 antibody
    • SKCa3 antibody
    • Small conductance calcium-activated potassium channel protein 3 antibody
    see all

Anti-KCNN3 antibody 图像

  • Sample Type : Rhesus macaque spinal cord. Primary Antibody Dilution : 1:300. Secondary Antibody : Donkey anti Rabbit 488. Secondary Antibody Dilution : 1:500. Color/Signal Descriptions : Green: KCNN3. Gene Name : KCNN3.
  • Anti-KCNN3 antibody (ab28631) at 2 µg/ml + Daudi (Human Burkitt’s lymphoma cell line) Whole Cell Lysate 10ug.

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size : 82 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)


  • Predicted band size : 82 kDa
    ab28631 at 2µg/ml used in Western blot. Negative control: mouse kidney lysate, positive sample: mouse whole brain lysate. The secondary antibody was goat anti-rabbit and used at 1/10,000 dilution.

Anti-KCNN3 antibody (ab28631)参考文献

ab28631 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (8% gel)
Sample Pig Cell lysate - whole cell (Endothelial Cell)
Specification Endothelial Cell
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Feb 27 2015

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