Anti-KCNN2抗体(ab99457)

概述

  • 产品名称Anti-KCNN2抗体
    参阅全部 KCNN2 一抗
  • 描述
    山羊多克隆抗体to KCNN2
  • 特异性Recognizes both Human isoforms (NP_067627.2 and NP_740721.1)
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Guinea pig, Cow, Dog, Orangutan
  • 免疫原

    Synthetic peptide: C-

    ESYDKHVTYNAER

    , corresponding to internal amino acids 546 - 557 of Human KCNN2 (NP_067627.2) and 197 - 209 of Human KCNN2 isoform b (NP_740721.1).

  • 阳性对照
    • Liver lysate Human thyroid

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab99457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70, 23 kDa (predicted molecular weight: 64, 26 kDa).Can be blocked with KCNN2 peptide (ab186680).

靶标

Anti-KCNN2 antibody 图像

  • Anti-KCNN2 antibody (ab99457) at 0.3 µg/ml + Human liver lysate, in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 64, 26 kDa
    Observed band size : 23,70 kDa (why is the actual band size different from the predicted?)
  • ab99457 at 3.8 µg/ml staining KCNN2 in Human thyroid by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody was revealed by AP-staining.

Anti-KCNN2 antibody (ab99457)参考文献

ab99457 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spinal cord)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate, pH6
Permeabilization Yes - 0.1% Tween-20
Specification spinal cord
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Mrs. Teresa Garrett

Verified customer

提交于 Oct 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"