概述

  • 产品名称
    Anti-KCNK2抗体
    参阅全部 KCNK2 一抗
  • 描述
    兔多克隆抗体to KCNK2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide within the N terminal region (amino acids 1-100) of Human KCNK2 (UniProt ID: Q9NRT2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Human brain membrane lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab90855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 47 kDa.
    It is essential to run against membrane preps.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 功能
      Outward rectifying potassium channel.
    • 序列相似性
      Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
      see all
    • 别名
      • hTREK 1c antibody
      • hTREK 1e antibody
      • K2p2.1 antibody
      • K2P2.1 potassium channel antibody
      • KCNK 2 antibody
      • Kcnk2 antibody
      • KCNK2_HUMAN antibody
      • MGC126742 antibody
      • MGC126744 antibody
      • Outward rectifying potassium channel protein TREK 1 antibody
      • Outward rectifying potassium channel protein TREK-1 antibody
      • Outward rectifying potassium channel protein TREK1 antibody
      • Potassium channel subfamily K member 2 antibody
      • Potassium inwardly rectifying channel subfamily K member 2 antibody
      • Tandem pore domain potassium channel TREK 1 antibody
      • Tandem pore domain potassium channel TREK1 antibody
      • TPKC1 antibody
      • TREK 1 antibody
      • TREK 1 K(+) channel subunit antibody
      • TREK antibody
      • TREK-1 K(+) channel subunit antibody
      • TREK1 antibody
      • TWIK related potassium channel 1 antibody
      • Two pore domain potassium channel TREK 1 antibody
      • Two pore domain potassium channel TREK-1 antibody
      • Two pore domain potassium channel TREK1 antibody
      • Two pore potassium channel 1 antibody
      • Two pore potassium channel TPKC1 antibody
      see all

    图片

    • Western blot - Anti-KCNK2 antibody (ab90855)
      Western blot - Anti-KCNK2 antibody (ab90855)
      Anti-KCNK2 antibody (ab90855) at 2 µg/ml + Human brain membrane lysate

      Predicted band size: 47 kDa

    实验方案

    文献

    This product has been referenced in:
    • Andharia N  et al. Involvement of intracellular transport in TREK-1c current run-up in 293T cells. Channels (Austin) 11:224-235 (2017). Read more (PubMed: 28085542) »
    • Bieling F  et al. Sepsis varies arterial two-pore-domain potassium channel messenger RNA in mice. J Surg Res 193:816-24 (2015). Read more (PubMed: 25300694) »

    See all 2 Publications for this product

    发表研究结果有使用 ab90855？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    5 reviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KCNK2 antibody

     Excellent
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (heart valve)
    Permeabilization
    Yes - tierton x
    Specification
    heart valve
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Hessah Alshammari

    Verified customer

    提交于 Nov 11 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-KCNK2 antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (heart valve)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    heart valve
    Blocking step
    endogenous peroxidases as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Hessah Alshammari

    Verified customer

    提交于 Nov 09 2016

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-KCNK2 antibody

     Good
    Abreviews
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain (motor cortex))
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    Brain (motor cortex)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Patrick Ye

    Verified customer

    提交于 Aug 11 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KCNK2 antibody

     Average
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 25°C
    Sample
    Human Cell (293 cells)
    Specification
    293 cells
    Permeabilization
    Yes - Tx-100
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Nov 04 2014

    I have a recombinant version of KCNK2 lacking a few amino acids in the amino terminus. Will this antibody still react?

    The antibody should still bind to the protein as its made from an immunogen containing the entire region of amino acids from 1-100.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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