Anti-KCNK18/TRESK抗体(ab96868)

概述

  • 产品名称Anti-KCNK18/TRESK抗体
    参阅全部 KCNK18/TRESK 一抗
  • 描述
    兔多克隆抗体to KCNK18/TRESK
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse KCNK18/TRESK.

    (Peptide available as ab109923.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Mouse forebrain; Mouse cerebellum; Mouse hippocampus; Mouse spinal cord; Mouse brain; Rat brain; Rat cortex.

性能

应用

Our Abpromise guarantee covers the use of ab96868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

靶标

  • 功能Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. May function as background potassium channel that sets the resting membrane potential. Channel activity is directly activated by calcium signal. Activated by the G(q)-protein coupled receptor pathway. The calcium signal robustly activates the channel via calcineurin, whereas the anchoring of 14-3-3/YWHAH interferes with the return of the current to the resting state after activation. Inhibited also by arachidonic acid and other naturally occurring unsaturated free fatty acids. Channel activity is also enhanced by volatile anesthetics, such as isoflurane. Appears to be the primary target of hydroxy-alpha-sanshool, an ingredient of Schezuan pepper. May be involved in the somatosensory function with special respect to pain sensation.
  • 组织特异性Expressed specifically in dorsal root ganglion and trigeminal ganglion neurons. Detected at low levels in spinal cord.
  • 疾病相关Defects in KCNK18 are a cause of migraine with or without aura type 13 (MGR13) [MIM:613656]. A form of migraine trasmitted in an autosomal dominant pattern. Migraine is a disabling symptom complex of periodic headaches, usually temporal and unilateral. Headaches are often accompanied by irritability, nausea, vomiting and photobia, preceded by constriction of the cranial arteries. The two major subtypes are common migraine (migraine without aura) and classic migraine (migraine with aura). Classic migraine is characterized by recurrent attacks of reversible neurological symptoms (aura) that precede or accompany the headache. Aura may include a combination of sensory disturbances, such as blurred vision, hallucinations, vertigo, numbness and difficulty in concentrating and speaking. Note=Susceptibility to migraine has been shown to be conferred by a frameshift mutation that segregates with the disorder in a large multigenerational family. Migraine was associated with sensitivity to lights, sounds, and smells, as well as nausea and occasional vomiting. Triggers included fatigue, alcohol and bright lights. Mutations in KCNK18 are a rare cause of migraine.
  • 序列相似性Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
  • 翻译后修饰Phosphorylation of Ser-252 is required for the binding of 14-3-3eta/YWHAH. Calcineurin-mediated dephosphorylation of Ser-264 enhances channel activity.
    N-glycosylated.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • K2p18.1 antibody
    • KCNK18 antibody
    • KCNKI_HUMAN antibody
    • MGR13 antibody
    • OTTHUMP00000020575 antibody
    • Potassium channel subfamily K member 18 antibody
    • TRESK 2 antibody
    • TRESK antibody
    • TRESK2 antibody
    • TRIK antibody
    • TWIK related individual K+ channel antibody
    • TWIK related individual potassium channel antibody
    • TWIK related spinal cord K+ channel antibody
    • TWIK related spinal cord potassium channel antibody
    • TWIK-related individual potassium channel antibody
    • TWIK-related spinal cord potassium channel antibody
    see all

Anti-KCNK18/TRESK antibody 图像

  • All lanes : Anti-KCNK18/TRESK antibody (ab96868) at 1 µg/ml

    Lane 1 : Forebrain (Mouse) Tissue Lysate
    Lane 2 : Cerebellum Mouse Tissue Lysate
    Lane 3 : Hippocampus (Mouse) Tissue Lysate
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate
    Lane 5 : Brain (Mouse) Tissue Lysate
    Lane 6 : Brain (Rat) Tissue Lysate
    Lane 7 : Rat Cortex Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 47 kDa,63 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-KCNK18/TRESK antibody (ab96868)参考文献

ab96868 has not yet been referenced specifically in any publications.

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