概述

  • 产品名称Anti-KCNJ5抗体
    参阅全部 KCNJ5 一抗
  • 描述
    兔多克隆抗体to KCNJ5
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KCNJ5 .

  • 阳性对照
    • This antibody gave a positive signal in Human Testis tissue lysate as well as the following whole cell lysates: A489; MCF7; A549. It also gave a positive signal in FFPE human pancreas tissue sections

性能

应用

Our Abpromise guarantee covers the use of ab113699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.
  • 组织特异性Islets, exocrine pancreas and heart.
  • 疾病相关Defects in KCNJ5 are the cause of long QT syndrome type 13 (LQT13) [MIM:613485]. It is a heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy.
  • 序列相似性Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cardiac ATP sensitive potassium channel antibody
    • Cardiac inward rectifier antibody
    • CIR antibody
    • G protein activated inward rectifier potassium channel 4 antibody
    • G protein-activated inward rectifier potassium channel 4 antibody
    • GIRK 4 antibody
    • GIRK-4 antibody
    • GIRK4 antibody
    • Heart KATP channel antibody
    • Inward rectifier K(+) channel Kir3.4 antibody
    • Inward rectifier K+ channel KIR3.4 antibody
    • inwardly rectifying subfamily J member 5 antibody
    • IRK-4 antibody
    • IRK5_HUMAN antibody
    • KATP 1 antibody
    • KATP-1 antibody
    • KATP1 antibody
    • KCNJ 5 antibody
    • Kcnj5 antibody
    • KIR 3.4 antibody
    • KIR3.4 antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 5 antibody
    • Potassium inwardly rectifying channel J5 antibody
    • Potassium inwardly rectifying channel subfamily J member 5 antibody
    see all

Anti-KCNJ5 antibody 图像

  • All lanes : Anti-KCNJ5 antibody (ab113699) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • IHC image of KCNJ5 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113699, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-KCNJ5 antibody (ab113699)参考文献

ab113699 has not yet been referenced specifically in any publications.

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