Anti-KCNE2抗体(ab69376)

概述

  • 产品名称Anti-KCNE2抗体
    参阅全部 KCNE2 一抗
  • 描述
    兔多克隆抗体to KCNE2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KCNE2.

    (Peptide available as ab86641.)

  • 阳性对照
    • This antibody gave a positive signal in mouse pancreas tissue lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab69376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Associated with KCNH2/HERG is proposed to form the rapidly activating component of the delayed rectifying potassium current in heart (IKr). May associate with KCNQ2 and/or KCNQ3 and modulate the native M-type current. May associate with KCNQ1/KVLTQ1 and elicit a voltage-independent current. May associate with HCN1 and HCN2 and increase potassium current.
  • 组织特异性Highly expressed in brain, heart, skeletal muscle, pancreas, placenta, kidney, colon and thymus. A small but significant expression is found in liver, ovary, testis, prostate, small intestine and leukocytes. Very low expression, nearly undetectable, in lung and spleen.
  • 疾病相关Defects in KCNE2 are the cause of long QT syndrome type 6 (LQT6) [MIM:613693]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. KCNE2 mutants form channels that open slowly and close rapidly, thereby diminishing potassium currents.
    Defects in KCNE2 are the cause of atrial fibrillation familial type 4 (ATFB4) [MIM:611493]. Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure.
  • 序列相似性Belongs to the potassium channel KCNE family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATFB4 antibody
    • cardiac voltage gated potassium channel accessory subunit 2 antibody
    • Kcne2 antibody
    • KCNE2_HUMAN antibody
    • LQT5 antibody
    • LQT6 antibody
    • minimum potassium ion channel related peptide 1 antibody
    • Minimum potassium ion channel-related peptide 1 antibody
    • minK related peptide 1 antibody
    • MinK-related peptide 1 antibody
    • MIRP1 antibody
    • Potassium channel subunit beta MiRP1 antibody
    • potassium channel subunit, MiRP1 antibody
    • potassium voltage gated channel subfamily E member 2 antibody
    • potassium voltage gated channel, Isk related family, member 2 antibody
    • Potassium voltage-gated channel subfamily E member 2 antibody
    • voltage-gated K+ channel subunit MIRP1 antibody
    see all

Anti-KCNE2 antibody 图像

  • Anti-KCNE2 antibody (ab69376) at 1 µg/ml + Pancreas (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Predicted band size : 14 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
  • IHC image of KCNE2 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69376, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-KCNE2 antibody (ab69376)参考文献

ab69376 has not yet been referenced specifically in any publications.

Product Wall

Indeed there are 2 glycosylation sites for this protein and if you prevent glycosylation of this protein then it should decrease the apparent size of the protein. However, glycosyl groups are small and therefore probably are not the cause for this high...

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If this band is indeed due to a dimerisation then I would sugest boiling the samples in SDS-PAge for 10 minutes to try and disrupt the formation of this multimer. Have you already tried this?
Is just one band being detected at 34 kDa? We have a bl...

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