Anti-KCNE1抗体(ab65795)

概述

  • 产品名称Anti-KCNE1抗体
    参阅全部 KCNE1 一抗
  • 描述
    兔多克隆抗体to KCNE1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from the N terminal domain of human KCNE1.

  • 阳性对照
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

性能

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应用

Our Abpromise guarantee covers the use of ab65795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/5000. Predicted molecular weight: 15 kDa.Can be blocked with KCNE1 peptide (ab151760).
IHC-P 1/100 - 1/1000.
ICC/IF 1/200.

靶标

  • 功能Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr).
  • 组织特异性Expressed in heart, lung, kidney, testis, ovaries, small intestine, peripheral blood leukocytes. Not detected in pancreas, spleen, prostate and colon. Restrictively localized in the apical membrane portion of epithelial cells.
  • 疾病相关Jervell and Lange-Nielsen syndrome 2 (JLNS2) [MIM:612347]: An autosomal recessive disorder characterized by congenital deafness, prolongation of the QT interval, syncopal attacks due to ventricular arrhythmias, and a high risk of sudden death. Note=The disease is caused by mutations affecting the gene represented in this entry.
    Long QT syndrome 5 (LQT5) [MIM:613695]: A heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • 序列相似性Belongs to the potassium channel KCNE family.
  • 翻译后修饰Phosphorylation inhibits the potassium current.
    N-glycosylation at Asn-26 occurs post-translationally, and requires prior cotranslational glycosylation at Asn-5.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Delayed rectifier potassium channel subunit IsK antibody
    • Human cardiac delayed rectifier potassium channel protein antibody
    • IKs producing slow voltage gated potassium channel subunit beta Mink antibody
    • IKs producing slow voltage-gated potassium channel subunit beta Mink antibody
    • ISK antibody
    • JLNS 2 antibody
    • JLNS antibody
    • JLNS2 antibody
    • KCNE 1 antibody
    • KCNE1 antibody
    • KCNE1_HUMAN antibody
    • LQT 5 antibody
    • LQT5 antibody
    • MGC33114 antibody
    • Minimal potassium channel antibody
    • MinK antibody
    • Potassium voltage gated channel Isk related family member 1 antibody
    • Potassium voltage gated channel Isk related subfamily member 1 antibody
    • Potassium voltage gated channel subfamily E member 1 antibody
    • Potassium voltage-gated channel subfamily E member 1 antibody
    see all

Anti-KCNE1 antibody 图像

  • ICC/IF image of ab65795 stained HepG2 cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab65795 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-KCNE1 antibody (ab65795)参考文献

ab65795 has not yet been referenced specifically in any publications.

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The blocking peptide for the rabbit polyclonal anti-KCNE1 is now available, the catalog reference number is ab151760 and the link to the datasheet is http://www.abcam.com/ab151760.

Unfortunately, the pepti...

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