Anti-KChIP2抗体(ab3473)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to KChIP2
  • 特异性
    Detects KChIP 2 b and c from rat and rabbit samples as well as recombinant rat KChIP 2c. This antibody does not detect KChIP 2a.
  • 经测试应用
    适用于: IHC-P, ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Dog, Non human primates
    预测可用于: Human
  • 免疫原

    Synthetic peptide corresponding to Human KChIP2 aa 6-24.
    Sequence:

    RKESLSDSRDLDGSYDQLT


    (Peptide available as ab4991)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    Voltage-gated potassium (Kv) channels are key components of the potassium currents in the heart and central nervous system. In the heart, Kv4 channels are involved in the repolarization phase of the action potential. In the brain however, these channels prevent reverse-propagation of action potentials. Found associated with Kv4 channels are a group of calcium-binding proteins termed KChIPs (Kv channel interacting proteins). KChIPs are small molecular weight proteins that bind to the cytoplasmic amino termini of Kv4 alpha-subunits and help to modulate its function. There are currently three known KChIPs; KChIP 1 expressed in the brain; KChIP 2 expressed in the brain, heart, and lung, and has three isoforms, a, b, and c; and KChIP 3, also known as calsenilin and DREAM (downstream regulatory elements (DRE)-antagonist modulator) which is expressed in the brain and testis.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Can be blocked with KChIP2 peptide (ab4991).

By Western blot, this antibody detects ~33 and 26 kDa proteins representing KChIP2b and c, respectively, from rat ventricular myocyte whole cell lysate.

靶标

  • 功能
    Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and KCND3 trafficking to the cell surface. May be required for the expression of I(To) currents in the heart.
  • 组织特异性
    Expressed in brain. Colocalizes with KCND2 in excitatory neurons including cortical and hippocampal CA1 pyramidal cells. Isoform 3 is expressed in heart and in umbilical vein endothelial cells. Not expressed in fetal heart.
  • 序列相似性
    Belongs to the recoverin family.
    Contains 4 EF-hand domains.
  • 翻译后修饰
    Palmitoylated. Palmitoylation enhances association with the plasma membrane.
  • 细胞定位
    Cell membrane and Cell membrane. Detected on lipid rafts (By similarity).
  • Information by UniProt
  • 数据库链接
  • 形式
    There are 9 isoforms produced by alternative splicing.
  • 别名
    • A type potassium channel modulatory protein 2 antibody
    • A-type potassium channel modulatory protein 2 antibody
    • Cardiac voltage gated potassium channel modulatory subunit antibody
    • Cardiac voltage-gated potassium channel modulatory subunit antibody
    • DKFZp566L1246 antibody
    • KChIP 2 antibody
    • KChIP2 antibody
    • KCIP2_HUMAN antibody
    • KCNIP 2 antibody
    • Kcnip2 antibody
    • Kv channel interacting protein 2 antibody
    • Kv channel-interacting protein 2 antibody
    • MGC17241 antibody
    • Potassium channel interacting protein 2 antibody
    • Potassium channel-interacting protein 2 antibody
    see all

Anti-KChIP2 antibody (ab3473)参考文献

ab3473 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (heart)
Loading amount
40 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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提交于 Apr 20 2007

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