概述

  • 产品名称Anti-KBTBD10抗体
    参阅全部 KBTBD10 一抗
  • 描述
    兔多克隆抗体to KBTBD10
  • 经测试应用适用于: IHC-Fr, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: AGSLYAIGGF AMIQLESKEF APTEVNDIWK YEDDKKEWAG MLKEIRYASG, corresponding to a region within C terminal amino acids 541-590 of Human KBTBD10

  • 阳性对照
    • Fetal muscle lysate

性能

应用

Our Abpromise guarantee covers the use of ab66605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 24268659
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Required for pseudopod elongation in transformed cells. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • 组织特异性Sarcomeric muscle.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 BTB (POZ) domain.
    Contains 5 Kelch repeats.
  • 翻译后修饰Ubiquitinated and probably targeted for proteasome-independent degradation.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Cell projection > pseudopodium. Cell projection > ruffle. Predominantly cytoplasmic but can co-localize with F-actin at the membrane ruffle-like structures at the tips of transformation-specific pseudopodia.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cb52 antibody
    • fb24f05 antibody
    • fc15e07 antibody
    • Gm112 antibody
    • KBTBA_HUMAN antibody
    • Kbtbd10 antibody
    • Kel like protein 23 antibody
    • Kel-like protein 23 antibody
    • Kelch related protein 1 antibody
    • Kelch repeat and BTB domain-containing protein 10 antibody
    • Kelch-related protein 1 antibody
    • KRP1 antibody
    • MGC143407 antibody
    • Sarcosin antibody
    • sbcb52 antibody
    • wufb24f05 antibody
    • wufc15e07 antibody
    see all

Anti-KBTBD10 antibody 图像

  • Immunohistochemistry (Frozen sections) analysis of human skeletal muscle tissue from control and nemaline myopathy affected individuals labelling KBTBD10 with ab66605. Costaining with sarcomeric markers in longitudinal planes showed that KLHL41 staining predominated over the I-bands of the sarcomere and at perinuclear regions. Scale bars represent 50 μm.

  • Anti-KBTBD10 antibody (ab66605) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    gel concentration: 12%

Anti-KBTBD10 antibody (ab66605)参考文献

This product has been referenced in:
  • Gupta VA  et al. Identification of KLHL41 Mutations Implicates BTB-Kelch-Mediated Ubiquitination as an Alternate Pathway to Myofibrillar Disruption in Nemaline Myopathy. Am J Hum Genet 93:1108-17 (2013). IHC-Fr ; Mouse, Human . Read more (PubMed: 24268659) »

See 1 Publication for this product

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