概述

  • 产品名称Anti-Kazrin抗体
    参阅全部 Kazrin 一抗
  • 描述
    兔多克隆抗体to Kazrin
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Kazrin.

  • 阳性对照
    • This antibody gave a positive signal in Kazrin over-expressing cell lines: HA tagged KazrinA and HA tagged KazrinE.

性能

应用

Our Abpromise guarantee covers the use of ab74114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 mg/ml. Detects a band of approximately 53,100 kDa (predicted molecular weight: 86 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Component of the cornified envelope of keratinocytes. May be involved in the interplay between adherens junctions and desmosomes. The function in the nucleus is not known.
  • 组织特异性Isoform 2, isoform 3 and isoform 4 are expressed in several cell lines including keratinocytes and bladder and epidermoid carcinoma (at protein level). Isoform 2, isoform 3 and isoform 4 are expressed in hair follicle and interfollicular epidermis (at protein level).
  • 序列相似性Belongs to the kazrin family.
    Contains 3 SAM (sterile alpha motif) domains.
  • 细胞定位Cytoplasm > cytoskeleton and Cytoplasm. Cell junction > desmosome. Nucleus. Observed at the apical plasma mebrane of keratinocytes. Partially colocalizes with PPL and DP at desmosomes, and with PP at the interdesmosomal plasma membrane. Colocalizes with cortical actin-based membrane structures.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HRIHFB2003 antibody
    • KAZ antibody
    • Kazn antibody
    • Kazrin antibody
    • KAZRN_HUMAN antibody
    • KIAA1026 antibody
    • RP1-21O18.1 antibody
    see all

Anti-Kazrin antibody 图像

  • All lanes : Anti-Kazrin antibody (ab74114) at 1 µg/ml

    Lane 1 : Over Expressing HA tagged KazrinA
    Lane 2 : Over Expressing HA tagged KazrinE

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 86 kDa
    Observed band size : 53,100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ICC/IF image of ab74114 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74114, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Kazrin antibody (ab74114)参考文献

ab74114 has not yet been referenced specifically in any publications.

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