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RabMAb

Anti-KATNA1抗体[EPR5071] (ab111881)

概述

  • 产品名称
    Anti-KATNA1抗体[EPR5071]
    参阅全部 KATNA1 一抗
  • 描述
    兔单克隆抗体[EPR5071] to KATNA1
  • 经测试应用
    适用于: WB, ICC/IFmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to residues from the C terminus of Human KATNA1.

  • 阳性对照
    • WB: HeLa and HepG2 cell lysates. ICC/IF: HeLa cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab111881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
ICC/IF 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能
      Severs microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays, such as during disassembly of interphase microtubules at the G2-M transition. May also be required for microtubule release from the centrosome after nucleation. In mitotic spindles this could allow depolymerization of the microtubule end proximal to the centrosome, and subsequent poleward microtubule flux. In neurons, microtubule release within the cell body may allow their subsequent transport into neuronal processes by microtubule dependent motor proteins. This transport is required for axonal growth.
    • 序列相似性
      Belongs to the AAA ATPase family.
    • 结构域
      The N-terminus is sufficient for interaction with microtubules, although high affinity binding requires an intact C-terminal domain and ATP, which promotes oligomerization.
    • 细胞定位
      Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Predominantly cytoplasmic. Also localized to the interphase centrosome. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Katanin p60 ATPase containing subunit A1 antibody
      • Katanin p60 ATPase-containing subunit A1 antibody
      • Katanin p60 subunit A1 antibody
      • KATNA1 antibody
      • KTNA1_HUMAN antibody
      • OTTHUMP00000017392 antibody
      • OTTHUMP00000042860 antibody
      • p60 katanin antibody
      see all

    图片

    • All lanes : Anti-KATNA1 antibody [EPR5071] (ab111881) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 56 kDa
    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KATNA1 with ab111881 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing staining on the centrosome of Hela cells.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:-

      -ve control 1: ab111881 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    文献

    ab111881 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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