Anti-KAT8 / MYST1 / MOF抗体(ab83502)


  • 产品名称Anti-KAT8 / MYST1 / MOF抗体
    参阅全部 KAT8 / MYST1 / MOF 一抗
  • 描述
    兔多克隆抗体to KAT8 / MYST1 / MOF
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KAT8/ MYST1/ MOF.

    (Peptide available as ab98900.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; A498; K562; A549; Caco 2




Our Abpromise guarantee covers the use of ab83502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa).


  • 功能Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM.
  • 序列相似性Belongs to the MYST (SAS/MOZ) family.
    Contains 1 C2HC-type zinc finger.
    Contains 1 chromo domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Histone acetyltransferase KAT8 antibody
    • Histone acetyltransferase MYST1 antibody
    • hMOF antibody
    • K(lysine) acetyltransferase 8 antibody
    • KAT 8 antibody
    • Lysine acetyltransferase 8 antibody
    • MOF antibody
    • MOZ antibody
    • MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1 antibody
    • MYST 1 antibody
    • MYST histone acetyltransferase 1 antibody
    • myst protein 1 antibody
    • MYST-1 antibody
    • MYST1 antibody
    • MYST1_HUMAN antibody
    • Ortholog of Drosophila males absent on the first (MOF) antibody
    • Probable histone acetyltransferase MYST1 antibody
    • SAS2 and TIP60 protein 1 antibody
    • SAS2 antibody
    • SAS3 antibody
    • TIP60 protein 1 antibody
    • YBF2 antibody
    • YBF2/SAS3 antibody
    • ZC2HC8 antibody
    see all

Anti-KAT8 / MYST1 / MOF antibody 图像

  • All lanes : Anti-KAT8 / MYST1 / MOF antibody (ab83502) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 5 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 7 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab83502 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83502, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and HepG2 cells at 1µg/ml.
  • IHC image of KAT8/MYST1/MOF staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab83502, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-KAT8 / MYST1 / MOF antibody (ab83502)参考文献

ab83502 has not yet been referenced specifically in any publications.

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