Anti-KAT5 / Tip60 (phospho S86)抗体(ab73207)
Key features and details
- Rabbit polyclonal to KAT5 / Tip60 (phospho S86)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KAT5 / Tip60 (phospho S86)抗体
参阅全部 KAT5 / Tip60 一抗 -
描述
兔多克隆抗体to KAT5 / Tip60 (phospho S86) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide corresponding to Human KAT5/ Tip60 (phospho S86).
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阳性对照
- Jurkat cell extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab73207 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73207于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
靶标
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功能
Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation. -
序列相似性
Belongs to the MYST (SAS/MOZ) family.
Contains 1 C2HC-type zinc finger. -
翻译后修饰
Sumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies.
Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity.
Ubiquitinated by MDM2, leading to its proteasome-dependent degradation. -
细胞定位
Nucleus > nucleolus. Cytoplasm > perinuclear region. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies. - Information by UniProt
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数据库链接
- Entrez Gene: 10524 Human
- Entrez Gene: 81601 Mouse
- Omim: 601409 Human
- SwissProt: Q92993 Human
- SwissProt: Q8CHK4 Mouse
- Unigene: 397010 Human
- Unigene: 228930 Mouse
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别名
- 60 kDa Tat interactive protein antibody
- 60 kDa Tat-interactive protein antibody
- cPLA(2) interacting protein antibody
see all
图片
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All lanes : Anti-KAT5 / Tip60 (phospho S86) antibody (ab73207) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Additional bands at: 35 kDa, 62 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab73207 被引用在 4 文献中.
- Hotokezaka Y et al. ATM-associated signalling triggers the unfolded protein response and cell death in response to stress. Commun Biol 3:378 (2020). PubMed: 32665601
- Nag D et al. Auranofin Protects Intestine against Radiation Injury by Modulating p53/p21 Pathway and Radiosensitizes Human Colon Tumor. Clin Cancer Res 25:4791-4807 (2019). PubMed: 30940656
- Li R et al. SUMO binding by the Epstein-Barr virus protein kinase BGLF4 is crucial for BGLF4 function. J Virol 86:5412-21 (2012). PubMed: 22398289
- Nayak G & Cooper GM p53 is a major component of the transcriptional and apoptotic program regulated by PI 3-kinase/Akt/GSK3 signaling. Cell Death Dis 3:e400 (2012). PubMed: 23059819