Anti-KAT4 / TBP Associated Factor 1抗体- ChIP Grade (ab28450)

概述

  • 产品名称Anti-KAT4 / TBP Associated Factor 1抗体- ChIP Grade
    参阅全部 KAT4 / TBP Associated Factor 1 一抗
  • 描述
    兔多克隆抗体to KAT4 / TBP Associated Factor 1 - ChIP Grade
  • 特异性ab28450 recognises transcription initiation factor TFIID subunit 1 (TAF1).
  • 经测试应用适用于: IHC-P, ELISA, WB, ChIPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Dog
  • 免疫原

    Synthetic peptide derived from residues 1823 - 1872 (C-terminal) of human TAF1.

  • 阳性对照
    • Daudi cell lysate, Human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab28450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/62500.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 212 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use 5-10 µg for 25 µg of chromatin.

靶标

  • 功能Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
  • 疾病相关Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
  • 序列相似性Belongs to the TAF1 family.
    Contains 2 bromo domains.
    Contains 1 HMG box DNA-binding domain.
    Contains 2 protein kinase domains.
  • 翻译后修饰Phosphorylated by casein kinase II in vitro.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BA2R antibody
    • CCG1 antibody
    • CCGS antibody
    • Cell cycle G1 phase defect antibody
    • Cell cycle gene 1 protein antibody
    • Complementation of cell cycle block G1 to S antibody
    • DYT3 antibody
    • N TAF1 antibody
    • NSCL2 antibody
    • OF antibody
    • p250 antibody
    • TAF 1 antibody
    • TAF(II)250 antibody
    • TAF1 antibody
    • TAF1 RNA polymerase II TATA box binding protein (TBP) associated factor 250kDa antibody
    • TAF1_HUMAN antibody
    • TAF2A antibody
    • TAFII-250 antibody
    • TAFII250 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD antibody
    • TBP associated factor 250 kDa antibody
    • TBP-associated factor 250 kDa antibody
    • Transcription factor TFIID p250 polypeptide antibody
    • Transcription initiation factor TFIID 250 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 1 antibody
    • XDP antibody
    see all

Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade 图像

  • Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

  • This image shows human kidney stained with ab28450 at 8µg/ml. Magnification 400x.
  • Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade (ab28450) at 0.5 µg/ml + Daudi cell lysate

    Predicted band size : 212 kDa
  • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28450 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
       

Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade (ab28450)参考文献

This product has been referenced in:
  • Iwata J  et al. Transforming growth factor-beta regulates basal transcriptional regulatory machinery to control cell proliferation and differentiation in cranial neural crest-derived osteoprogenitor cells. J Biol Chem 285:4975-82 (2010). WB ; Mouse . Read more (PubMed: 19959467) »
  • van Essen D  et al. Two modes of transcriptional activation at native promoters by NF-kappaB p65. PLoS Biol 7:e73 (2009). ChIP ; Mouse . Read more (PubMed: 19338389) »

See all 2 Publications for this product

Product Wall

Application ChIP
Sample Mouse Cell lysate - nuclear (ESCs)
Specification ESCs
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde 1 % (37ºC)
Positive control samples
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提交于 Sep 15 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human fibromatosis)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Permeabilization Yes - Tween-20
Specification Human fibromatosis
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
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Abcam user community

Verified customer

提交于 Apr 15 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Sample Dog Tissue sections (MDCK pellets in paraffin)
Specification MDCK pellets in paraffin
Permeabilization Yes - Tween-20
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 04 2015

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. This antibody has been applied by Abcams chromatin postdoc scientist by adding 5-10µg of antibody to 25µg of chromatin. Please can you tell me ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"