Anti-KAT4 / TBP Associated Factor 1抗体(ab24427)

概述

  • 产品名称Anti-KAT4 / TBP Associated Factor 1抗体
    参阅全部 KAT4 / TBP Associated Factor 1 一抗
  • 描述
    小鼠多克隆抗体to KAT4 / TBP Associated Factor 1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Fusion protein:

    LSNDDKYQILKKTHQTKVRSTISNLNIQHSQPAINLQSPFYKVAVPRYQL RHFHRENFGSHIRPGTKIVFSKLKARKRKRDKGKDVKESFSTSQDLTIGD

    , corresponding to amino acids 441/540 of Saccharomyces cerevisiae TAF1

  • 常规说明Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

应用

Our Abpromise guarantee covers the use of ab24427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 120 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
  • 疾病相关Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
  • 序列相似性Belongs to the TAF1 family.
    Contains 2 bromo domains.
    Contains 1 HMG box DNA-binding domain.
    Contains 2 protein kinase domains.
  • 翻译后修饰Phosphorylated by casein kinase II in vitro.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • BA2R antibody
      • CCG1 antibody
      • CCGS antibody
      • Cell cycle G1 phase defect antibody
      • Cell cycle gene 1 protein antibody
      • Complementation of cell cycle block G1 to S antibody
      • DYT3 antibody
      • N TAF1 antibody
      • NSCL2 antibody
      • OF antibody
      • p250 antibody
      • TAF 1 antibody
      • TAF(II)250 antibody
      • TAF1 antibody
      • TAF1 RNA polymerase II TATA box binding protein (TBP) associated factor 250kDa antibody
      • TAF1_HUMAN antibody
      • TAF2A antibody
      • TAFII-250 antibody
      • TAFII250 antibody
      • TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD antibody
      • TBP associated factor 250 kDa antibody
      • TBP-associated factor 250 kDa antibody
      • Transcription factor TFIID p250 polypeptide antibody
      • Transcription initiation factor TFIID 250 kDa subunit antibody
      • Transcription initiation factor TFIID subunit 1 antibody
      • XDP antibody
      see all

    Anti-KAT4 / TBP Associated Factor 1 antibody 图像

    • All lanes : Anti-KAT4 / TBP Associated Factor 1 antibody (ab24427) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
      Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 120 kDa

    Anti-KAT4 / TBP Associated Factor 1 antibody (ab24427)参考文献

    ab24427 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application ChIP
    Sample Saccharomyces cerevisiae Cell lysate - whole cell (no case)
    Specification no case
    Type Cross-linking (X-ChIP)
    Duration of cross-linking step: 60 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde final concentr
    Detection step Real-time PCR
    Positive control The given gene in Active conditions and a very strong constitutive gene TEF1.
    Negative control The given gene at INACTIVE conditions and a 3" UTR of the same gene at ACTIVE conditions
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    提交于 Nov 22 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"