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Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab14984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21705428|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 300 kDa).
We recommend using 3% milk as the blocking agent in Western Blot.
|ChIP||Use 2µg for 106 cells.|
|IHC-Fr||Use at an assay dependent concentration.|
The recruitment of p300 to the genes that exhibited changes in H3K56ac was analyzed using ChIP analysis, with IL-8 as a positive control and Mef2c, GATA4, and Nkx2.5 as negative controls.
ChIP was performed on HeLa chromatin from 4 million cells using 8ug of ab14984. Positive control locus IRS2 shows enrichment over the negative control MyoD as expected.
This data was provided by an anonymous collaborator.
CBX7 promotes p300-PTEN promoter binding and Histone H3 acetylation in pancreatic cancer cells. CTRL, CBX7, and CBX7i Panc-1 cells were subjected to CHIP assay to determine the enrichment of p300 and acetylated Histone H3 on PTEN promoter. (*,# P<0.05, compared with the control group.)
ChIP assay was performed using a ChIP qPCR Assay Kit. The bound PTEN promoter sequence was verified using RT-PCR (Real-Time polymerase chain reaction) assay with DNA fragments immunoprecipitated by anti-p300 antibodies (ab14984), anti-acetyl-histone H3, or control rabbit IgG. The DNA fragments were amplified by quantitative RT-PCR. The RT-PCR primers are 5′-CGG GCG GTG ATG TGG C-3′ and 5′-GCC TCA CAG CGG CTC AAC TCT-3′.
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