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Synthetic peptide corresponding to Human KAT3A/ CBP aa 162-176.
Our Abpromise guarantee covers the use of ab2832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).Can be blocked with Human KAT3A / CBP peptide (ab4916).|
|ChIP||Use at an assay dependent concentration. PubMed: 20111703
Recommended to be used at 2-4µg for 1-2x106 cells.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration. PubMed: 20403362|
|CHIPseq||Use at an assay dependent concentration. PubMed: 22196736|
|Inhibition Assay||Use at an assay dependent concentration.|
Sonicated chromatin prepared from U2OS cells was subjected to the ChIP procedure with ab2832 to CBP. Immunoprecipitated chromatin was analysed in the promoter region of c-FOS (active) and in exon 2 of MYO-D (inactive). Values are % of inputs. 2–4 µg of ab2832 and 1-2x106 cells were used in each ChIP experiment.
Sergei Denissov, Prof. Henk Stunnenberg lab, University Nijmengen, NL
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