Anti-KAT3A / CBP抗体- ChIP Grade (ab10490)

概述

  • 产品名称Anti-KAT3A / CBP抗体- ChIP Grade
    参阅全部 KAT3A / CBP 一抗
  • 描述
    兔多克隆抗体to KAT3A / CBP - ChIP Grade
  • 经测试应用适用于: IP, ChIP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human CREB binding protein encoded within exon 33 (LocusLink ID 1387)(between residues 2392 and the c-terminus (residue 2442) according to Swiss-Prot entry Q92793).

  • 常规说明


    Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.

性能

应用

Our Abpromise guarantee covers the use of ab10490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 1-4 µg/mg of lysate.
ChIP Use 2-4µg for 106 cells.
WB 1/1000 - 1/25000. Predicted molecular weight: 265 kDa.Can be blocked with Human KAT3A / CBP peptide (ab4916).
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
  • 疾病相关Note=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
    Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
  • 序列相似性Contains 1 bromo domain.
    Contains 1 KIX domain.
    Contains 2 TAZ-type zinc fingers.
    Contains 1 ZZ-type zinc finger.
  • 结构域The KIX domain mediates binding to HIV-1 Tat.
  • 翻译后修饰Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.
  • 细胞定位Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CBP antibody
    • CBP_HUMAN antibody
    • CREB binding protein antibody
    • CREB-binding protein antibody
    • Crebbp antibody
    • Cyclic AMP responsive enhancer binding protein antibody
    • KAT3A antibody
    • RSTS antibody
    • RTS antibody
    • Rubinstein Taybi syndrome antibody
    see all

Anti-KAT3A / CBP antibody - ChIP Grade 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling KAT3A / CBP with ab10490 at 1/500 (2µg/ml). Detection: DAB.
  • ab10490 immunoprecipitation of CREB Binding Protein.

    Samples: Nuclear (NE) or cytoplasmic (S100) extract (10 mg) from HeLa cells. Antibody: ab10490 used at 20 µg/10 mg extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is CREB Binding Protein.

  • Sonicated chromatin prepared from U2OS cells was subjected to the ChIP procedure with ab10490 to CBP. Immunoprecipitated chromatin was analysed in the promoter region of c-FOS (active) and in exon 2 of MYO-D (inactive), values are % of inputs.  2–4 µg of ab10490 and 1-2x106 cells were used in each ChIP experiment.

    Sergei Denissov, Prof. Henk Stunnenberg lab, University Nijmengen, NL

Anti-KAT3A / CBP antibody - ChIP Grade (ab10490)参考文献

ab10490 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. We have the following antibodies in our catalog that where tested in ChIP: for CBP: ab2832, ab10489, and ab10490 for p300: ab14984 None of the antibodies has been tested so far in zebrafish. If you ...

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