The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IP: Use at a concentration of 2-5 µg/ml.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL.
组织特异性Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
ab93805 at 1 µg/ml detecting KAT13D/CLOCK in HeLa whole cell lysate by WB following IP.
Lane 1: ab93805 at 3µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of KAT13D/CLOCK
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
Anti-KAT13D / CLOCK antibody (ab93805)参考文献
has not yet been referenced specifically in any publications.