The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at a concentration of 2-5 µg/ml.
WB: 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL.
Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
ab93804 at 1 µg/ml detecting KAT13D/CLOCK in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of KAT13D/CLOCK
Lane 2: ab93804 at 3µg/mg of lysate
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds