Anti-KAT13C / NCOA2抗体- ChIP Grade (ab9261)

概述

  • 产品名称
    Anti-KAT13C / NCOA2抗体- ChIP Grade
    参阅全部 KAT13C / NCOA2 一抗
  • 描述
    兔多克隆抗体to KAT13C / NCOA2 - ChIP Grade
  • 经测试应用
    适用于: IHC-P, IP, WB, ChIPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH - which represents aportion between residues 600 and 700 of the human Nuclear Receptor Coactivator 2 (GenBank PID 1877215).

性能

应用

Our Abpromise guarantee covers the use of ab9261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 1 - 4 µg/ml.
WB 1/500 - 1/5000. Predicted molecular weight: 159 kDa.
ChIP Use at an assay dependent concentration.

靶标

  • 功能
    Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues.
  • 疾病相关
    Note=Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation.
  • 序列相似性
    Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 bHLH (basic helix-loop-helix) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • 结构域
    Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.
    The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
    Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHe75 antibody
    • Class E basic helix-loop-helix protein 75 antibody
    • glucocorticoid receptor interacting protein 1 antibody
    • GRIP 1 antibody
    • GRIP1 antibody
    • hTIF2 antibody
    • MED1 antibody
    • MGC138808 antibody
    • NCoA 2 antibody
    • NCoA-2 antibody
    • Ncoa2 antibody
    • NCOA2_HUMAN antibody
    • Nuclear receptor coactivator 2 antibody
    • Oncogene FGR antibody
    • p160 antibody
    • SRC 2 antibody
    • src2 antibody
    • Steroid receptor coactivator 2 antibody
    • TIF 2 antibody
    • TIF2 antibody
    • Transcriptional intermediary factor 2 antibody
    see all

Anti-KAT13C / NCOA2 antibody - ChIP Grade 图像

  • ab9261 (2µg/ml) staining KAT13C in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-KAT13C / NCOA2 antibody - ChIP Grade (ab9261)参考文献

This product has been referenced in:
  • Madsen A  et al. Metformin inhibits hepatocellular glucose, lipid and cholesterol biosynthetic pathways by transcriptionally suppressing steroid receptor coactivator 2 (SRC-2). Sci Rep 5:16430 (2015). ChIP . Read more (PubMed: 26548416) »
  • Wagner M  et al. NCOA3 is a selective co-activator of estrogen receptor a-mediated transactivation of PLAC1 in MCF-7 breast cancer cells. BMC Cancer 13:570 (2013). ChIP ; Human . Read more (PubMed: 24304549) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7)
Loading amount
10000 cells
Specification
huh-7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

提交于 Jul 15 2008

This antibody has never been tested in mouse or rat, so we can't guarantee results.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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