概述

  • 产品名称Anti-KAT13C / NCOA2抗体
    参阅全部 KAT13C / NCOA2 一抗
  • 描述
    兔多克隆抗体to KAT13C / NCOA2
  • 特异性We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    corresponding to KAT13C/ NCOA2.
    Database link: Q15596

  • 阳性对照
    • Nuclear or cytosolic extract from HeLa cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab10508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 1-4 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 159 kDa.

靶标

  • 功能Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues.
  • 疾病相关Note=Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation.
  • 序列相似性Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 bHLH (basic helix-loop-helix) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • 结构域Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.
    The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
    Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHe75 antibody
    • Class E basic helix-loop-helix protein 75 antibody
    • glucocorticoid receptor interacting protein 1 antibody
    • GRIP 1 antibody
    • GRIP1 antibody
    • hTIF2 antibody
    • MED1 antibody
    • MGC138808 antibody
    • NCoA 2 antibody
    • NCoA-2 antibody
    • Ncoa2 antibody
    • NCOA2_HUMAN antibody
    • Nuclear receptor coactivator 2 antibody
    • Oncogene FGR antibody
    • p160 antibody
    • SRC 2 antibody
    • src2 antibody
    • Steroid receptor coactivator 2 antibody
    • TIF 2 antibody
    • TIF2 antibody
    • Transcriptional intermediary factor 2 antibody
    see all

Anti-KAT13C / NCOA2 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling KAT13C / NCOA2 with ab10508 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-KAT13C / NCOA2 antibody (ab10508) at 1 µg/ml

    Lane 1 : TCMK-1 whole cell lysate
    Lane 2 : 4T1 whole cell lysate
    Lane 3 : CT26.WT whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 159 kDa


    Exposure time : 3 minutes
  • ab10508 immunoprecipitation of human NCOA2 (SRC2).  Samples: Nuclear extract (A, 10 mg) or cytosolic (S100) extract (B, 10 mg) from HeLa cells. Antibody: ab10508 used at 20 µg/10 mg lysate. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2 (SRC2).

Anti-KAT13C / NCOA2 antibody (ab10508)参考文献

This product has been referenced in:
  • Zhang Y  et al. A systems biology-based classifier for hepatocellular carcinoma diagnosis. PLoS One 6:e22426 (2011). IHC-P ; Human . Read more (PubMed: 21829460) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab10508.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"