Anti-KAT13A / SRC1抗体- ChIP Grade (ab2859)

概述

  • 产品名称Anti-KAT13A / SRC1抗体- ChIP Grade
    参阅全部 KAT13A / SRC1 一抗
  • 描述
    兔多克隆抗体to KAT13A / SRC1 - ChIP Grade
  • 特异性Detects steroid receptor coactivator-1 (SRC-1).
  • 经测试应用适用于: IHC-Fr, IHC-P, Flow Cyt, ChIP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human, Bird
    预测可用于: Pig
  • 免疫原

    Synthetic peptide corresponding to Human KAT13A/ SRC1 aa 8-24.
    Sequence:

    SSDPANPDSHKRKGSPC


    Database link: Q15788
    (Peptide available as ab5848)

  • 阳性对照
    • HeLa cell lysate

相关产品

应用

Our Abpromise guarantee covers the use of ab2859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 3-5µg for 106 cells.
ChIP Use at an assay dependent concentration. PubMed: 19005469
ICC/IF 1/200.

靶标

  • 功能Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
  • 组织特异性Widely expressed.
  • 疾病相关Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
  • 序列相似性Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • 结构域The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
    Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.
  • 翻译后修饰Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
    Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHe74 antibody
    • Class E basic helix-loop-helix protein 74 antibody
    • F SRC 1 antibody
    • Hin 2 protein antibody
    • Hin2 protein antibody
    • MGC129719 antibody
    • MGC129720 antibody
    • mNRC 1 antibody
    • NCoA 1 antibody
    • NCoA-1 antibody
    • Ncoa1 antibody
    • NCOA1_HUMAN antibody
    • Nuclear receptor coactivator 1 antibody
    • Nuclear receptor coactivator protein 1 antibody
    • NY REN 52 antigen antibody
    • Protein Hin 2 antibody
    • Protein Hin-2 antibody
    • Protein Hin2 antibody
    • Renal carcinoma antigen NY REN 52 antibody
    • Renal carcinoma antigen NY-REN-52 antibody
    • RIP 160 antibody
    • RIP160 antibody
    • SRC 1 antibody
    • SRC-1 antibody
    • Steroid receptor coactivator 1 antibody
    see all

Anti-KAT13A / SRC1 antibody - ChIP Grade 图像

  • Immunolocalization of SRC1 in HeLa cells using ab2859.

  • ab2859 at 1/2000 staining mouse brain tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 72 hours. A biotinylated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Immunohistochemitry (Frozen sections) analysis of SRC1 in the Dentate Gyrus region of the rat brain using ab2859.

  • Flow cytometry analysis of SRC1 was done on HT-29 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature.Cells were labeled with SRC1 Rabbit Polyclonal Antibody (PA1840, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature.

Anti-KAT13A / SRC1 antibody - ChIP Grade (ab2859)参考文献

This product has been referenced in:
  • Hurtado A  et al. Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen. Nature 456:663-6 (2008). WB, ChIP ; Human . Read more (PubMed: 19005469) »
  • Yasumoto H  et al. GNL3L inhibits activity of estrogen-related receptor gamma by competing for coactivator binding. J Cell Sci 120:2532-43 (2007). WB . Read more (PubMed: 17623774) »

See all 2 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (huh-7 cells)
Loading amount 10000 cells
Specification huh-7 cells
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Aug 11 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX-100 in PBS for 10 min at 4'C
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 37°C
Username

Dr. Sungsoo Lee

Verified customer

提交于 May 19 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Jiqiang Zhang

Verified customer

提交于 Jun 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1%
Username

Dr. Jiqiang Zhang

Verified customer

提交于 May 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"