Anti-KAT13A / SRC1抗体(ab5407)

概述

  • 产品名称
    Anti-KAT13A / SRC1抗体
    参阅全部 KAT13A / SRC1 一抗
  • 描述
    兔多克隆抗体to KAT13A / SRC1
  • 经测试应用
    适用于: WB, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human KAT13A/ SRC1 aa 600-650.
    Database link: Q15788

应用

Our Abpromise guarantee covers the use of ab5407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000.
IHC-P 1/500 - 1/2000.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能
    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
  • 组织特异性
    Widely expressed.
  • 疾病相关
    Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
  • 序列相似性
    Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • 结构域
    The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
    Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.
  • 翻译后修饰
    Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
    Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHe74 antibody
    • Class E basic helix-loop-helix protein 74 antibody
    • F SRC 1 antibody
    • Hin 2 protein antibody
    • Hin2 protein antibody
    • MGC129719 antibody
    • MGC129720 antibody
    • mNRC 1 antibody
    • NCoA 1 antibody
    • NCoA-1 antibody
    • Ncoa1 antibody
    • NCOA1_HUMAN antibody
    • Nuclear receptor coactivator 1 antibody
    • Nuclear receptor coactivator protein 1 antibody
    • NY REN 52 antigen antibody
    • Protein Hin 2 antibody
    • Protein Hin-2 antibody
    • Protein Hin2 antibody
    • Renal carcinoma antigen NY REN 52 antibody
    • Renal carcinoma antigen NY-REN-52 antibody
    • RIP 160 antibody
    • RIP160 antibody
    • SRC 1 antibody
    • SRC-1 antibody
    • Steroid receptor coactivator 1 antibody
    see all

图片

  • All lanes : Anti-KAT13A / SRC1 antibody (ab5407) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 5 µg
    Lane 4 : HeLa cells at 50 µg
    Lane 5 : Mouse NIH3T3 cells at 50 µg

    Detection:Chemiluminescence

    Exposure time:30 seconds

  • KAT13A/SRC1  was immunoprecipitated from 1mg of 293T cells prepared using NETN lysis buffer.

    Exposure time: 10 seconds.

  • Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma labeling KAT13A/SRC1 with ab5407 at 1/1000 dilution.

    Detection: DAB.

    Counter stained with Hematoxylin.

文献

This product has been referenced in:
  • Du M  et al. Transgenic Mice Overexpressing Serum Retinol-Binding Protein Develop Progressive Retinal Degeneration through a Retinoid-Independent Mechanism. Mol Cell Biol 35:2771-89 (2015). Read more (PubMed: 26055327) »

See 1 Publication for this product

客户评价及客户问答

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ab84; http://www.abcam.com/KAT13A-SRC1-antibody-1135-H4-ChIP-Grade-ab84.html

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Tissue lysate - nuclear (huh-7)
Total protein in input
1e+006 cells
Specification
huh-7
Treatment
cells expressing recombitant protein
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

提交于 Aug 23 2009

The peptide comes from upstream of aa 627. It is not contained within aa 627 to 786 according to Q15788 (Swiss-Prot entry). If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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