Anti-KAT1 / HAT1抗体(ab12163)

概述

• 产品名称
  Anti-KAT1 / HAT1抗体
  参阅全部 KAT1 / HAT1 一抗
• 描述
  兔多克隆抗体to KAT1 / HAT1
• 特异性
  ab12163 specifically recognizes human HAT1.
• 经测试应用
  适用于: WBmore details
• 种属反应性
  与反应: Mouse, Chicken, Human
  
• 免疫原

  Synthetic peptide:

  EESFQELVEDYRRVIERLAQE

  with N-terminal added lysine conjugated to KLH, corresponding to amino acids 399-419 of Human HAT1. The corresponding sequence differs by three amino acids in chicken and by one amino acid in mouse (putative sequence).

  Run BLAST with Run BLAST with
• 阳性对照
  • Whole extracts of human 293 cells, mouse NIH-3T3 cells, or chicken fibroblasts.
• 常规说明
  Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• 存储溶液
  Preservative: 15mM Sodium Azide
  Constituents: 1% BSA, 0.01M PBS, pH 7.4
• Concentration information loading...
• 纯度
  Immunogen affinity purified
• 纯化说明
  The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
• 克隆
  多克隆
• 同种型
  IgG
• 研究领域
  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HAT

相关产品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Positive Controls
  • HEK293 whole cell lysate (ab7902)

应用

靶标

• 功能
  Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
• 序列相似性
  Belongs to the HAT1 family.
• 发展阶段
  Highly expressed in mitotic cells (at protein level).
• 细胞定位
  Nucleus matrix and Cytoplasm. Nucleus. Nucleus matrix. Nucleus > nucleoplasm. Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.

• Target information above from: UniProt accession O14929 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 374037 Chicken
  • Entrez Gene: 8520 Human
  • Entrez Gene: 107435 Mouse
  • Omim: 603053 Human
  • SwissProt: O14929 Human
  • SwissProt: Q8BY71 Mouse
  • Unigene: 632532 Human
  • Unigene: 272472 Mouse
• 别名
  • HAT 1 antibody
  • hat1 antibody
  • HAT1_HUMAN antibody

  • Histidine aminotransferase 1 antibody
  • Histone acetyltransferase 1 antibody
  • Histone acetyltransferase 1 antibody
  • Histone acetyltransferase type B catalytic subunit antibody
  • KAT1 antibody

  see all

实验方案

• Western blot protocols