Anti-Karyopherin beta 3抗体(ab88695)

概述

  • 产品名称Anti-Karyopherin beta 3抗体
    参阅全部 Karyopherin beta 3 一抗
  • 描述
    小鼠多克隆抗体to Karyopherin beta 3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length human Karyopherin (importin) beta 3 protein (AAH45640.1).

  • 阳性对照
    • WB: Rat brain tissue lysate; HeLa cell lysate; Karyopherin (importin) beta 3 transfected 293T cell lysate ICC/IF: HeLa cell

性能

应用

Our Abpromise guarantee covers the use of ab88695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 124 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
  • 序列相似性Belongs to the importin beta family.
    Contains 6 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • 细胞定位Cytoplasm. Nucleus. Nucleus > nucleolus. Nucleus; nuclear rim. Found particularly in the nuclear rim and nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686O1576 antibody
    • FLJ43041 antibody
    • IMB3 antibody
    • Imp5 antibody
    • Importin 5 antibody
    • Importin beta 3 subunit antibody
    • Importin subunit beta 3 antibody
    • Importin subunit beta-3 antibody
    • Importin-5 antibody
    • IPO 5 antibody
    • IPO5 antibody
    • IPO5_HUMAN antibody
    • Karyopherin beta 3 antibody
    • Karyopherin beta-3 antibody
    • KPNB3 antibody
    • MGC2068 antibody
    • Pse1 antibody
    • Ran binding protein 5 antibody
    • Ran GTP binding protein 5 antibody
    • Ran-binding protein 5 antibody
    • RanBP 5 antibody
    • RanBP5 antibody
    see all

Anti-Karyopherin beta 3 antibody 图像

  • Anti-Karyopherin beta 3 antibody (ab88695) at 1 µg/ml + rat brain tissue lysate at 50 µg

    Predicted band size : 124 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • Anti-Karyopherin beta 3 antibody (ab88695) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 124 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Karyopherin beta 3 antibody (ab88695) at 1 µg/ml

    Lane 1 : Karyopherin (importin) beta 3 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 124 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
  • ab88695, at 10 µg/ml, staining Karyopherin (importin) beta 3 in HeLa cell by immunofluorescence.

Anti-Karyopherin beta 3 antibody (ab88695)参考文献

ab88695 has not yet been referenced specifically in any publications.

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Unfortunately, all of our antibodies to Karyopherin beta 3 are raised against the human protein, which is not very homologous with your protein of interest. I would not expect any of them to show any significant s...

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