Anti-Kappa Opioid Receptor抗体(ab113533)

概述

  • 产品名称Anti-Kappa Opioid Receptor抗体
    参阅全部 Kappa Opioid Receptor 一抗
  • 描述
    兔多克隆抗体to Kappa Opioid Receptor
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide within Human Kappa Opioid Receptor aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P41145
    (Peptide available as ab138734)

  • 阳性对照
    • This antibody gave a positive signal in HEK293 whole cell lysates as well as the following Human tissue lysates: Brain; Spinal Cord; Placenta; Small Intestine. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: SKNSH This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab113533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • K-OR-1 antibody
    • Kappa opioid receptor antibody
    • Kappa type opioid receptor antibody
    • Kappa-type opioid receptor antibody
    • KOR 1 antibody
    • KOR antibody
    • KOR-1 antibody
    • Opiate receptor kappa 1 antibody
    • Opiate receptor, kappa 1 antibody
    • Opioid receptor, kappa 1 antibody
    • OPRK 1 antibody
    • OPRK antibody
    • OPRK_HUMAN antibody
    • OPRK1 antibody
    • Oprk2 antibody
    • R21 antibody
    see all

Anti-Kappa Opioid Receptor antibody 图像

  • All lanes : Anti-Kappa Opioid Receptor antibody (ab113533) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Human placenta tissue lysate - total protein (ab29745)
    Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 20 kDa,33 kDa,38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ab113533 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113533 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of Kappa Opioid Receptor staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113533, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Kappa Opioid Receptor antibody (ab113533)参考文献

ab113533 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (spinal cord)
Gel Running Conditions Reduced Denaturing (10%)
Specification spinal cord
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Username

Ms. Louise Faggionato Kimura Vieira

Verified customer

提交于 Jul 06 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"