Anti-Kappa free light chains抗体[5H11] (ab54312)

概述

  • 产品名称Anti-Kappa free light chains抗体[5H11]
    参阅全部 Kappa free light chains 一抗
  • 描述
    小鼠单克隆抗体[5H11] to Kappa free light chains
  • 经测试应用适用于: ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Mixture of light chains of human immunoglobulin.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号5H11
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab54312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 数据库链接
  • 别名
    • Free kappa light chain antibody
    • Free kappa light chains antibody
    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • Immunoglobulin kappa constant antibody
    • Kappa free light chain antibody
    • Kappa light chain free antibody
    • Kappa light chains free antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Anti-Kappa free light chains antibody [5H11] 图像

  • Overlay histogram showing Ramos cells stained with ab54312 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54312, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Kappa free light chains antibody [5H11] (ab54312)参考文献

ab54312 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Pig Cell (blood cells)
Permeabilization No
Gating Strategy Lymphoid gate only
Specification blood cells
Preparation Cell harvesting/tissue preparation method: hypotonic lysis of erythrocytes
Sample buffer: phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin from Cold Water Fish Skin (PBS-GEL)
Fixation None
Username

Dr. Marek Sinkora

Verified customer

提交于 Jun 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"