Anti-Kappa free light chains抗体[5H11] (ab54312)

概述

  • 产品名称
    Anti-Kappa free light chains抗体[5H11]
    参阅全部 Kappa free light chains 一抗
  • 描述
    小鼠单克隆抗体[5H11] to Kappa free light chains
  • 经测试应用
    适用于: ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Mixture of light chains of human immunoglobulin.

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    5H11
  • 同种型
    IgG2b
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab54312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 数据库链接
  • 别名
    • Free kappa light chain antibody
    • Free kappa light chains antibody
    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • Immunoglobulin kappa constant antibody
    • Kappa free light chain antibody
    • Kappa light chain free antibody
    • Kappa light chains free antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Anti-Kappa free light chains antibody [5H11] 图像

  • Overlay histogram showing Ramos cells stained with ab54312 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54312, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Kappa free light chains antibody [5H11] (ab54312)参考文献

ab54312 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Pig Cell (blood cells)
Permeabilization
No
Gating Strategy
Lymphoid gate only
Specification
blood cells
Preparation
Cell harvesting/tissue preparation method: hypotonic lysis of erythrocytes
Sample buffer: phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin from Cold Water Fish Skin (PBS-GEL)
Fixation
None
Username

Dr. Marek Sinkora

Verified customer

提交于 Jun 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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