A synthetic phosphorylated peptide, corresponding to a sequence of human KAP1 surrounding Serine 824. NP_005753.1
Our Abpromise guarantee covers the use of ab70369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
ICC | Use at an assay dependent concentration. | |
WB | 1/1000 - 1/5000. Detects a band of approximately 117 kDa (predicted molecular weight: 89 kDa). | |
ICC/IF | 1/1000. | |
IP | Use at 2-5 µg/mg of lysate. |
Detection of Human KAP1 (phospho S824) by Western Blot of Immunoprecipitates.
Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells that were mock treated (-) or treated with Etoposide (100 µM, 2h).
Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of KAP1 (phospho S824) by ab70369 (1:200) in NBF-fixed HeLa cells by Immunocytochemistry.
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