- 数据表
- 具体的参考文献 (11)
- 实验方案
概述
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产品名称Anti-KAP1 (phospho S824)抗体
参阅全部 KAP1 一抗 -
描述兔多克隆抗体to KAP1 (phospho S824)
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经测试应用适用于: ICC, WB, ICC/IF, IPmore details
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种属反应性与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
A synthetic phosphorylated peptide, corresponding to a sequence of human KAP1 surrounding Serine 824. NP_005753.1
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阳性对照
- Whole cell lysate of asynchronous HeLa cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab70369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/5000. Detects a band of approximately 117 kDa (predicted molecular weight: 89 kDa). | |
| ICC/IF | 1/1000. | |
| IP | Use at 2-5 µg/mg of lysate. |
靶标
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功能Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.
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组织特异性Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
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通路Protein modification; protein sumoylation.
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序列相似性Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger. -
结构域The HP1 box is both necessary and sufficient for HP1 binding.
The PHD-type zinc finger enhances CEBPB transcriptional activity. The PHD-type zinc finger, the HP1 box and the bromo domain, function together to assemble the machinery required for repression of KRAB domain-containing proteins. Acts as an intramolecular SUMO E3 ligase for autosumoylation of bromodomain.
The RING-finger-B Box-coiled-coil/tripartite motif (RBCC/TRIM motif) is required for interaction with the KRAB domain of KRAB-zinc finger proteins. Binds four zinc ions per molecule. The RING finger and the N-terminal of the leucine zipper alpha helical coiled-coil region of RBCC are required for oligomerization.
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. -
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR. ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes.
Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21. -
细胞定位Nucleus. Associated with centromeric heterochromatin during cell differentiation through CBX1.
- Information by UniProt
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数据库链接
- Entrez Gene: 10155 Human
- Entrez Gene: 21849 Mouse
- Entrez Gene: 711982 Rhesus monkey
- Omim: 601742 Human
- SwissProt: Q13263 Human
- SwissProt: Q62318 Mouse
- Unigene: 467408 Human
- Unigene: 15701 Mouse
see all -
别名
- E3 SUMO protein ligase TRIM28 antibody
- E3 SUMO-protein ligase TRIM28 antibody
- FLJ29029 antibody
see all
Anti-KAP1 (phospho S824) antibody 图像
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Immunoprecipitation - Anti-KAP1 (phospho S824) antibody (ab70369)Detection of Human KAP1 (phospho S824) by Western Blot of Immunoprecipitates.
Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells that were mock treated (-) or treated with Etoposide (100 µM, 2h).
Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody (ab70369)Detection of KAP1 (phospho S824) by ab70369 (1:200) in NBF-fixed HeLa cells by Immunocytochemistry.
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Western blot - Anti-KAP1 (phospho S824) antibody (ab70369)All lanes : Anti-KAP1 (phospho S824) antibody (ab70369) at 0.1 µg/ml
Lane 1 : Whole cell lysate from asynchronous HeLa cells, treated with neocarzinostatin (NCS; 200 ng/ml, 30 minutes) at 50 µg
Lane 2 : Whole cell lysate from asynchronous HeLa cells at 50 µg
Lane 3 : Whole cell lysate from asynchronous HeLa cells, treated with neocarzinostatin (NCS; 200 ng/ml, 30 minutes) at 200 µg
Lane 4 : Whole cell lysate from asynchronous HeLa cells at 200 µg
Developed using the ECL technique
Predicted band size : 89 kDa
Observed band size : 117 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody (ab70369)This image is courtesy of an Abreview submitted by Ms. Can Zhouab70369 staining KAP1 (phospho S824) (red) in Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were treated with 1 µM Etoposide for 3 hours prior to fixing. Cells were fixed with formaldehyde, permeabilized in 0.5% NP40 and blocked with 3% BSA for 2 hours at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 3% BSA) for 12 hours at 4°C. An Alexa Fluor®594-conjugated Mouse anti-rabbit IgG monoclonal (1/500) was used as the secondary antibody.
实验方案
Anti-KAP1 (phospho S824) antibody (ab70369)参考文献
This product has been referenced in:
- Deneka AY et al. Tumor-targeted SN38 inhibits growth of early stage non-small cell lung cancer (NSCLC) in a KRas/p53 transgenic mouse model. PLoS One 12:e0176747 (2017). IHC ; Mouse . Read more (PubMed: 28453558) »
- Gaponova AV et al. A Novel HSP90 Inhibitor-Drug Conjugate to SN38 Is Highly Effective in Small Cell Lung Cancer. Clin Cancer Res 22:5120-5129 (2016). Read more (PubMed: 27267850) »
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