重组
RabMAb

Anti-KAP1抗体[EPR5216] (ab109287)

概述

  • 产品名称
    Anti-KAP1抗体[EPR5216]
    参阅全部 KAP1 一抗
  • 描述
    兔单克隆抗体[EPR5216] to KAP1
  • 经测试应用
    适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab109287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 89 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.
    • 组织特异性
      Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
    • 通路
      Protein modification; protein sumoylation.
    • 序列相似性
      Belongs to the TRIM/RBCC family.
      Contains 2 B box-type zinc fingers.
      Contains 1 bromo domain.
      Contains 1 PHD-type zinc finger.
      Contains 1 RING-type zinc finger.
    • 结构域
      The HP1 box is both necessary and sufficient for HP1 binding.
      The PHD-type zinc finger enhances CEBPB transcriptional activity. The PHD-type zinc finger, the HP1 box and the bromo domain, function together to assemble the machinery required for repression of KRAB domain-containing proteins. Acts as an intramolecular SUMO E3 ligase for autosumoylation of bromodomain.
      The RING-finger-B Box-coiled-coil/tripartite motif (RBCC/TRIM motif) is required for interaction with the KRAB domain of KRAB-zinc finger proteins. Binds four zinc ions per molecule. The RING finger and the N-terminal of the leucine zipper alpha helical coiled-coil region of RBCC are required for oligomerization.
      Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
    • 翻译后修饰
      Phosphorylated upon DNA damage, probably by ATM or ATR. ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes.
      Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21.
    • 细胞定位
      Nucleus. Associated with centromeric heterochromatin during cell differentiation through CBX1.
    • Information by UniProt
    • 数据库链接
    • 别名
      • E3 SUMO protein ligase TRIM28 antibody
      • E3 SUMO-protein ligase TRIM28 antibody
      • FLJ29029 antibody
      • KAP 1 antibody
      • KAP-1 antibody
      • KRAB associated protein 1 antibody
      • KRAB interacting protein 1 antibody
      • KRAB-associated protein 1 antibody
      • KRAB-interacting protein 1 antibody
      • KRIP 1 antibody
      • KRIP-1 antibody
      • KRIP1 antibody
      • Nuclear corepressor KAP 1 antibody
      • Nuclear corepressor KAP-1 antibody
      • RING finger protein 96 antibody
      • RNF96 antibody
      • TF1B antibody
      • TIF1 beta antibody
      • TIF1-beta antibody
      • TIF1B antibody
      • TIF1B_HUMAN antibody
      • Transcription intermediary factor 1 beta antibody
      • Transcription intermediary factor 1-beta antibody
      • Trim28 antibody
      • Tripartite motif containing 28 antibody
      • tripartite motif containing protein 28 antibody
      • Tripartite motif-containing protein 28 antibody
      see all

    Anti-KAP1 antibody [EPR5216] 图像

    • All lanes : Anti-KAP1 antibody [EPR5216] (ab109287) at 1/10000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : PC-3 cell lysates
      Lane 4 : MCF7 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 89 kDa
    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using 1/250 ab109287.
    • Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/250 ab109287.
    • Immunofluorescent staining of HeLa cells using 1/100 ab109287.

    Anti-KAP1 antibody [EPR5216] (ab109287)参考文献

    ab109287 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (vascular smooth muscle cell)
    Permeabilization
    Yes - NP40
    Specification
    vascular smooth muscle cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Nov 04 2015

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cell)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    100000 cells
    Specification
    mouse embryonic stem cell
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 20 2015

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF7 cell line)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    100000 cells
    Specification
    MCF7 cell line
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 18 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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