antigen sequence, corresponding to amino acids 377-526 of Human KANK4.
Human colon tissue; RT-4 and U-251 MG cell lysates; Human liver and tonsil tissue lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Contains 5 ANK repeats.
Information by UniProt
Ankyrin repeat domain 38 antibody
Ankyrin repeat domain-containing protein 38 antibody
Immunocytochemistry/ Immunofluorescence - Anti-KANK4 antibody (ab121410)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and cytoskeleton (microtubules). Recommended concentration of ab121410 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KANK4 antibody (ab121410)
ab121410 at 1/50 dilution staining KANK4 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Western blot - Anti-KANK4 antibody (ab121410)
All lanes : Anti-KANK4 antibody (ab121410) at 1/250 dilution Lane 1 : RT-4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human plasma Lane 4 : Human liver tissue lysate Lane 5 : Human tonsil tissue lysate Developed using the ECL technique.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"