概述

  • 产品名称
    Anti-KALRN抗体
  • 描述
    山羊多克隆抗体to KALRN
  • 特异性
    This antibody is expected to recognize isoform 2 (NP_003938.1).
  • 经测试应用
    适用于: WB, ELISA, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide:

    C-RWHLGPGDPFSTYV

    , corresponding to C terminal amino acids 1650-1663 of Human KALRN

  • 阳性对照
    • Mouse Brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab52012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 192 kDa).
ELISA Use at an assay dependent concentration. Peptide ELISA: antibody detection limit dilution 1:8000.
IHC-Fr 1/200.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity.
  • 组织特异性
    Isoform 2 is brain specific. Highly expressed in cerebral cortex, putamen, amygdala, hippocampus and caudate nucleus. Weakly expressed in brain stem and cerebellum. Isoform 4 is expressed in skeletal muscle.
  • 疾病相关
    Genetic variation in KALRN is associated with susceptibility to coronary heart disease type 5 (CHDS5) [MIM:608901]. CHD is the leading cause of death and disability worldwide. CHD is multifactorial disease with a strong genetic component. Classic epidemiologic studies have revealed many risk factors for CHD, including age, sex, hypertension, dyslipidemia, diabetes mellitus, smoking, and physical inactivity.
  • 序列相似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 CRAL-TRIO domain.
    Contains 2 DH (DBL-homology) domains.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 2 PH domains.
    Contains 1 protein kinase domain.
    Contains 2 SH3 domains.
    Contains 5 spectrin repeats.
  • 结构域
    The two GEF domains catalyze nucleotide exchange for RAC1 and RhoA which are bound by DH1 and DH2 respectively. The two GEF domains appear to play differing roles in neuronal development and axonal outgrowth. SH3 1 binds to the first GEF domain inhibiting GEF activity only when in the presence of a PXXP peptide, suggesting that the SH3 domain/peptide interaction mediates binding to GEF1. CRK1 SH3 domain binds to and inhibits GEF1 activity.
  • 翻译后修饰
    Autophosphorylated.
  • 细胞定位
    Cytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Duo antibody
    • FLJ16443 antibody
    • HAPIP antibody
    • Huntingtin associated protein interacting protein (duo) antibody
    • Huntingtin-associated protein-interacting protein antibody
    • Kalirin (isoform 2) antibody
    • Kalirin antibody
    • KALRN antibody
    • KALRN_HUMAN antibody
    • Protein Duo antibody
    • RhoGEF kinase antibody
    • Serine/threonine kinase with Dbl and pleckstrin homology domains antibody
    • Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain antibody
    • TRAD antibody
    see all

图片

  • ab52012 staining KALRN in adult brain tissue by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step performed using citrate buffer. Tissues were then blocked with 15% serum for 45 minutes at 20°C followed by incubation with the primary antibody, at a 1/250 dilution, for 24 hours at 4°C. A Biotin-conjugated rabbit anti-goat IgG was used as secondary antibody at a 1/250 dilution.
    Upper image: Un-treated.

    Lower image: Treated with peptide to KALRN.

    Counterstained with Hämalaun.

    See Abreview

  • All lanes : Anti-KALRN antibody (ab52012) at 1/500 dilution

    Lane 1 : Whole tissue lysate prepared from mouse brain, treated with peptide.
    Lane 2 : Whole cell lysate prepared from mouse brain cells over-expressing KALRN (with FLAG-tag) treated with peptide.
    Lane 3 : Whole cell lysate prepared from mouse brain cells over-expressing KALRN, with FLAG-tag, detected with anti-FLAG antibody.
    Lane 4 : Whole tissue lysate prepared from mouse brain
    Lane 5 : Whole cell lysate prepared from mouse brain cells over-expressing KALRN, with FLAG-tag.

    Lysates/proteins at 30 µg per lane.

    Secondary
    Donkey anti-goat IgG conjugated to HRP at 1/10000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 192 kDa
    Observed band size : 192 kDa


    Exposure time : 1 minute

    Image kindly supplied by Dr Janine Magg through Abreview

    See Abreview

  • Anti-KALRN antibody (ab52012) at 0.5 µg/ml + Mouse Brain lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 192 kDa

文献

This product has been referenced in:
  • Afroz S  et al. Synaptic pruning in the female hippocampus is triggered at puberty by extrasynaptic GABAA receptors on dendritic spines. Elife 5:N/A (2016). ICC . Read more (PubMed: 27136678) »
  • Lewis MA  et al. Exploring regulatory networks of miR-96 in the developing inner ear. Sci Rep 6:23363 (2016). Read more (PubMed: 26988146) »

See all 3 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Whole brain sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - 0.05% Tween-20
Specification
Whole brain sections
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Jun 02 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain tissue lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-8% Bis-Tris)
Loading amount
20 µg
Specification
Whole brain tissue lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

提交于 Jun 02 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (Lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Lung
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Apr 11 2017

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 11 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 11 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 11 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney)
Permeabilization
Yes - 0.2% triton x100
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 24 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (kidney)
Permeabilization
Yes - 0.2% triton x100
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 24 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% triton x100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 24 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (mouse spinal cord, 20 micron)
Specification
mouse spinal cord, 20 micron
Permeabilization
Yes - Triton X-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 11 2014

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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