Anti-Kaiso抗体[6F / 6F8] - ChIP Grade (ab12723)

概述

  • 产品名称Anti-Kaiso抗体[6F / 6F8] - ChIP Grade
    参阅全部 Kaiso 一抗
  • 描述
    小鼠单克隆抗体[6F / 6F8] to Kaiso - ChIP Grade
  • 经测试应用适用于: Flow Cyt, IHC-P, ChIP, ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Hamster, Dog, Human
  • 免疫原

    His-tagged Kaiso fusion protein (Human) lacking the zinc finger region.

  • 阳性对照
    • CSML0 cells (IP then Western). In Flow Cytometry, this antibody gave a positive signal in HeLa cells.
  • 常规说明

    We can conjugate this antibody to FITC for you (please see ab150256 for details).

性能

应用

Our Abpromise guarantee covers the use of ab12723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
IP Use at an assay dependent concentration.

靶标

  • 功能Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.
  • 组织特异性Expressed in vascular endothelium.
  • 序列相似性Contains 1 BTB (POZ) domain.
    Contains 3 C2H2-type zinc fingers.
  • 细胞定位Nucleus. Cytoplasm. Also cytoplasmic in cells grown at high densities.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Kaiso antibody
    • Kaiso protein antibody
    • Kaiso transcription factor antibody
    • KAISO_HUMAN antibody
    • Transcriptional regulator Kaiso antibody
    • ZBTB 33 antibody
    • Zbtb33 antibody
    • Zinc finger and BTB domain containing 33 antibody
    • Zinc finger and BTB domain-containing protein 33 antibody
    • Zinc finger transcription factor Kaiso antibody
    • ZNF 348 antibody
    • ZNF Kaiso antibody
    • ZNF348 antibody
    see all

Anti-Kaiso antibody [6F / 6F8] - ChIP Grade 图像

  • Immunofluorescence using clone 6F / 6F8 on C2C12 cells.


  • Predicted band size : 80 kDa

    Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.

    Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.
  • Overlay histogram showing HeLa cells stained with ab12723 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12723, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunofluroesence analysis of Human ARPE-19 cells, staining Kaiso using ab12723.


  • Predicted band size : 80 kDa

    Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9.; Fig 4.; doi:10.1371/journal.pone.0036864; May 9, 2012, PLoS ONE 7(5): e36864.

    Representative western blot detecting Kaiso in ARPE-19 cells using ab12723.

Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)参考文献

This product has been referenced in:
  • Zhou L  et al. Kaiso represses the expression of glucocorticoid receptor via a methylation-dependent mechanism and attenuates the anti-apoptotic activity of glucocorticoids in breast cancer cells. BMB Rep 49:167-72 (2016). Human . Read more (PubMed: 26424557) »
  • Liu Y  et al. Kaiso interacts with p120-catenin to regulate ß-catenin expression at the transcriptional level. PLoS One 9:e87537 (2014). IP, ChIP ; Human . Read more (PubMed: 24498333) »

See all 10 Publications for this product

Product Wall


It seems that this antibody is sometimes referred to as 6F8 and sometimes as 6F. So we've labeled it with both clone names.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Human Cell (SW480)
Specification SW480
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Mouse Cell (NIH 3T3)
Specification NIH 3T3
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (sw480)
Specification sw480
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mice APC Min)
Specification Mice APC Min
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris buffer
Permeabilization No
Blocking step 1% gelatin 10%FBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Mr. Raul Peña

Verified customer

提交于 Dec 18 2012

Thank you for contacting Abcam and for letting us know that ab12723 was used in that publication. I have passed this information to our references team, who will review the article and then upload that reference to our website.
If there is anythin...

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Thank you for your inquiry. I heard back from our lab that unfortunately we do not have any more information on the immunogen sequence other than what is stated on the datasheet.    Ideally,  for an antibody that works in ChIP, the immunogen se...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreas)
Specification Pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Apr 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"