Anti-Kaiso抗体[2T15] (ab11810)

概述

  • 产品名称Anti-Kaiso抗体[2T15]
    参阅全部 Kaiso 一抗
  • 描述
    小鼠单克隆抗体[2T15] to Kaiso
  • 经测试应用适用于: ICC, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Dog
    预测可用于: Chicken, Human
  • 免疫原

    Recombinant fusion protein (N-terminal), corresponding to amino acids 103 - 584 of mouse Kaiso.

  • 阳性对照
    • 3T3/A31 cell lysate. Add 2.5µl of 2-mercaptoethanol/100µl of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
  • 常规说明For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

性能

应用

Our Abpromise guarantee covers the use of ab11810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
EMSA
ICC
WB
IP
  • 应用说明GSA: Use at an assay dependent dilution. Supershifts a specific methyl-CpG complex.
    ICC: Use at a concentration of 1 µg/ml for positive nuclear staining in NIH3T3 cells fixed with 100% methanol.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 2 µg/ml on RIPA lysates from 3T3/A31 cells (ECL). Detects a band of approximately 97 kDa (predicted molecular weight: 75 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.
    • 组织特异性Expressed in vascular endothelium.
    • 序列相似性Contains 1 BTB (POZ) domain.
      Contains 3 C2H2-type zinc fingers.
    • 细胞定位Nucleus. Cytoplasm. Also cytoplasmic in cells grown at high densities.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Kaiso antibody
      • Kaiso protein antibody
      • Kaiso transcription factor antibody
      • KAISO_HUMAN antibody
      • Transcriptional regulator Kaiso antibody
      • ZBTB 33 antibody
      • Zbtb33 antibody
      • Zinc finger and BTB domain containing 33 antibody
      • Zinc finger and BTB domain-containing protein 33 antibody
      • Zinc finger transcription factor Kaiso antibody
      • ZNF 348 antibody
      • ZNF Kaiso antibody
      • ZNF348 antibody
      see all

    Anti-Kaiso antibody [2T15] (ab11810)参考文献

    ab11810 has not yet been referenced specifically in any publications.

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