概述

  • 产品名称
  • 描述
    兔多克隆抗体to KA1
  • 经测试应用
    适用于: WB, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 60-73 of Human KA1.

  • 阳性对照
    • Mouse lung lysate.

性能

应用

Our Abpromise guarantee covers the use of ab10101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19114596

靶标

  • 功能
    Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.
  • 序列相似性
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK4 subfamily.
  • 细胞定位
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EAA 1 antibody
    • EAA1 antibody
    • Excitatory amino acid receptor 1 antibody
    • GluK4 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic kainate 4 antibody
    • Glutamate receptor ionotropic kainate 4 precursor antibody
    • Glutamate receptor KA 1 antibody
    • Glutamate receptor KA 1precursor antibody
    • Glutamate receptor KA-1 antibody
    • Glutamate receptor KA1 antibody
    • Glutamate receptor, ionotropic kainate 4 antibody
    • GRIK 4 antibody
    • GRIK antibody
    • Grik4 antibody
    • GRIK4_HUMAN antibody
    • ionotropic kainate 4 antibody
    • KA 1 antibody
    • KA1 antibody
    • OTTHUMP00000045951 antibody
    • OTTHUMP00000231881 antibody
    see all

图片

  • ICC/IF image of ab10101 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10101, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-KA1 antibody (ab10101) at 1/1000 dilution + Mouse lung

    Predicted band size : 107 kDa

文献

This product has been referenced in:
  • Gu L  et al. Erythropoietin exerts a neuroprotective function against glutamate neurotoxicity in experimental diabetic retina. Invest Ophthalmol Vis Sci 55:8208-22 (2014). Read more (PubMed: 25335981) »
  • Vargas JR  et al. The expression of kainate receptor subunits in hippocampal astrocytes after experimentally induced status epilepticus. J Neuropathol Exp Neurol 72:919-32 (2013). WB . Read more (PubMed: 24042195) »

See all 3 Publications for this product

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