K562全细胞裂解液(ab29306)

概述

  • 产品名称K562全细胞裂解液
    参阅全部 K562 细胞裂解液
  • 常规说明Cell line: K562 (Human erythromyeloblastoid leukemia).


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.

  • 经测试应用适用于: WBmore details

性能

  • 背景K-562 is an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. Cells are also used for haemoglobin synthesis, antitumour testing, tumorigenicity, differentiation, pharmacodynamics, cloning, cytotoxicity, cell biology, cellular effects of hyperthermia, and natural killer assays.

应用

Our Abpromise guarantee covers the use of ab29306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

K562 whole cell lysate (ab29306)参考文献

ab29306 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I suggest increasing the primary antibody concentration based on the images which show poor bands for the expected size (˜95kDa)as compared to the 70kDa non specific band. I also suggest using BSA as a blocking...

Read More

Thank you for contacting us.

The lysates ab29306 and ab7911 were prepared using slightly different techniques, but will give equivalent results in WB. Either of these lysates will be guaranteed for use as a positive control with ab93373. We r...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"