K562膜extract裂解液(ab29308)

概述

  • 产品名称K562膜extract裂解液
    参阅全部 K562 细胞裂解液
  • 常规说明Cell line: K562 (Human erythromyeloblastoid leukemia).


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • 存储溶液Preservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • 裂解物说明Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • 研究领域
  • 背景K-562 is an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. Cells are also used for haemoglobin synthesis, antitumour testing, tumorigenicity, differentiation, pharmacodynamics, cloning, cytotoxicity, cell biology, cellular effects of hyperthermia, and natural killer assays.

应用

Our Abpromise guarantee covers the use of ab29308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

K562 membrane extract lysate (ab29308)参考文献

ab29308 has not yet been referenced specifically in any publications.

Product Wall

Thank you for confirming this information and for your help and cooperation with this case.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order...

Read More

Thank you for your feedback.

I am very sorry that the suggestions did not resolve the problem andthat the customer isnot satisfied with the lysate. I would however appreciate if we could take the time to investigate this issue some further. A...

Read More

Thank you for taking time to complete our questionnaire. I am sorry to hear that thislysate is not providing satisfactory results.

Having reviewed this case, I would like to address the following:

Depending on the gel buffers, the ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"