The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
应用说明Is unsuitable for WB.
功能E3 UFM1-protein ligase that mediates ufmylation of target proteins such as DDRGK1/C20orf116. The function of ufmylation is unknown. May act as a tumor suppressor by inhibiting cell invasion, blocking NF-kappa-B signaling and increasing stability of CDK5RAP3.
组织特异性Ubiquitously expressed, with a high expression in liver (at protein level). Low expression in several invasive hepatocellular carcinomas, such HepG2, Hep3B, HLE and PLC.
序列相似性Belongs to the UFL1 family.
翻译后修饰Ubiquitinated, leading to its degradation by the proteasome. Interaction with CDK5RAP3 protects each other against ubiquitination and degradation via the proteasome.
细胞定位Endoplasmic reticulum. Cytoplasm. In cytoplasm colocalizes with CDK5RAP3.
Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123500 for IP at 6 µg/mg lysate. Subsequent Western blot detection used an antibody which recognized a downstream epitope of K0776 at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Anti-K0776 antibody (ab123500)参考文献
has not yet been referenced specifically in any publications.