Jurkat核extract裂解物(TPA + CI stimulated) (ab14850)
概述
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产品名称
Jurkat核extract裂解物(TPA + CI stimulated)
参阅全部 Jurkat 细胞裂解液 -
常规说明
Cell line: Jurkat (Human T cell lymphoblast-like).
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12-myristate, 13 acetate) and 0.5 µM calcium ionophore A23187 (CI) for two hours at 37°C immediately prior to harvesting.
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading...
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研究领域
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背景
Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab14850 尚未被引用在任何文献中。