Jurkat核extract裂解液(TPA + CI stimulated) (ab14850)

概述

  • 产品名称Jurkat核extract裂解液(TPA + CI stimulated)
    参阅全部 Jurkat 细胞裂解液
  • 描述
    Jurkat核extract裂解液
  • 常规说明Cell line: Jurkat (Human T cell lymphoblast-like).
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
    Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12-myristate, 13 acetate) and 0.5 µM calcium ionophore A23187 (CI) for two hours at 37°C immediately prior to harvesting.

性能

  • 形式Liquid
  • 存放说明Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • 研究领域
  • 背景Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.

Jurkat nuclear extract lysate (TPA + CI stimulated) (ab14850)参考文献

ab14850 has not yet been referenced specifically in any publications.

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