Jurkat核extract裂解液(ab14844)

概述

  • 产品名称Jurkat核extract裂解液
    参阅全部 Jurkat 细胞裂解液
  • 常规说明Cell line: Jurkat (Human T cell lymphoblast-like).
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • 研究领域
  • 背景Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.

Jurkat nuclear extract lysate (ab14844)参考文献

ab14844 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. We probed for nuclear markers, mainly transcription factors such as ATF-2, E12/47, GATA-2, NF-yB, Tal-1, USF, SP1, AML-1. Unfortunately there is no data available showing the absence of cytoplasmic markers. I hope this...

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Thank you for your enquiry and your interest in our products. Ab14844 is Jurkat nuclear lysate and you need to dilute it in a standard Western blot sample buffer. It may be worth boiling the sample for 1 or 2 minutes before loading. Usually for ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"