Cow, Dog, Chimpanzee
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human JunD.
(Peptide available as ab74427.)
- This antibody gave a positive signal in the following Human Nuclear Lysates:
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 35 kDa).
Transcription factor binding AP-1 sites.
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain.
Information by UniProt
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
Western blot - Anti-JunD antibody (ab74428)
All lanes :
Anti-JunD antibody (ab74428) at 1 µg/mlLane 1 :
HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 43 kDa (why is the actual band size different from the predicted?)
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The 43 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to JunD.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"