Mouse, Rat, Chicken, Cow, Dog, Chimpanzee
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human JunD.
(Peptide available as
This antibody gave a positive signal in the following Human Lysates:
HeLa Nuclear, Jurkat Nuclear, HEK293 Whole Cell, HepG2 Nuclear
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 35 kDa).
Anti-JunD antibody 图像
Western blot - JunD antibody (ab67453)
All lanes :
Anti-JunD antibody (ab67453) at 1 µg/ml
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate ( ab14844) Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 4 : HepG2 nuclear extract lysate ( ab14660) Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 35 kDa Observed band size : 43 kDa ( why is the actual band size different from the predicted?) Additional bands at : 130 kDa,37 kDa. We are unsure as to the identity of these extra bands. Exposure time : 16 minutes
Immunocytochemistry/ Immunofluorescence - JunD antibody (ab67453)
ICC/IF image of ab67453 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67453, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and HepG2 cells at 1µg/ml.
Anti-JunD antibody (ab67453)参考文献
has not yet been referenced specifically in any publications.
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