概述

  • 产品名称Anti-JunD抗体
    参阅全部 JunD 一抗
  • 描述
    兔多克隆抗体to JunD
  • 特异性ab28837 recognises JunD.
  • 经测试应用适用于: IHC-P, WB, ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of Serine 255.

  • 阳性对照
    • Breast carcinoma, extracts from Hela cells.

性能

应用

Our Abpromise guarantee covers the use of ab28837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
ELISA 1/20000.
IP Use at an assay dependent concentration.

靶标

Anti-JunD antibody 图像

  • Lane 1 : Anti-JunD antibody (ab28837) at 1/500 dilution
    Lane 2 : Anti-JunD antibody (ab28837) at 1/500 dilution (preincubated with synthesized peptide.)

    Lane 1 : extracts from Hela cells.
    Lane 2 : extracts from Hela cells.


    Predicted band size : 35 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded breast carcinoma, using 1/50 JunD Antibody(ab28837).

Anti-JunD antibody (ab28837)参考文献

This product has been referenced in:
  • Suwei D  et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . Read more (PubMed: 26503334) »
  • Tili E  et al. Resveratrol decreases the levels of miR-155 by upregulating miR-663, a microRNA targeting JunB and JunD. Carcinogenesis 31:1561-6 (2010). WB ; Human . Read more (PubMed: 20622002) »

See all 3 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Other - EMSA
Sample Human Cell lysate - nuclear (renal cancer cells)
Specification renal cancer cells
Total protein in input 3 µg
Username

Abcam user community

Verified customer

提交于 Mar 14 2014

Thank you for your inquiry. Firstly, this antibody is not phospho-specific. It will recognize the total protein. The immunogen is a synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of Serine 255. We g...

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Thank you for contacting us. The antibody was raised against a sequence around Serine 255 of human JunD. It is not a phospho antibody, though, and will bind the protein only if it is NOT phosphorylated at S255. Regarding the 2 isoforms: ...

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Thank you for contacting us. All our product are covered by Abpromise guarantee which states we replace or fully refund products not performing as stated on the datasheet when reported within 6 months of delivery. Please click the follow...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"