Anti-Junctional Adhesion Molecule 1抗体(ab125886)

概述

  • 产品名称
    Anti-Junctional Adhesion Molecule 1抗体
    参阅全部 Junctional Adhesion Molecule 1 一抗
  • 描述
    兔多克隆抗体to Junctional Adhesion Molecule 1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Chinese hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Junctional Adhesion Molecule 1.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cortex; Rat Cortex; Mouse Cerebellum; Mouse Spinal Cord.

性能

应用

Our Abpromise guarantee covers the use of ab125886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 32 kDa).

靶标

  • 功能
    Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
  • 序列相似性
    Belongs to the immunoglobulin superfamily.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • 翻译后修饰
    N-glycosylated.
  • 细胞定位
    Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 321 antibody
    • CD321 antibody
    • CD321 antigen antibody
    • ESTM33 antibody
    • F11 receptor antibody
    • F11R antibody
    • JAM 1 antibody
    • JAM A antibody
    • JAM antibody
    • JAM-1 antibody
    • JAM-A antibody
    • JAM1 antibody
    • JAM1_HUMAN antibody
    • JAMA antibody
    • JCAM antibody
    • Jcam1 antibody
    • Junction adhesion molecule 1 antibody
    • Junction adhesion molecule, mouse, homolog of antibody
    • Junctional adhesion molecule 1 antibody
    • Junctional adhesion molecule A antibody
    • KAT antibody
    • Ly106 antibody
    • PAM 1 antibody
    • PAM-1 antibody
    • PAM1 antibody
    • Platelet adhesion molecule 1 antibody
    • Platelet adhesion molecule antibody
    • Platelet F11 receptor antibody
    • PRO301 antibody
    • UNQ264 antibody
    see all

图片

  • All lanes : Anti-Junctional Adhesion Molecule 1 antibody (ab125886) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Mouse Cortex Tissue Lysate
    Lane 4 : Rat Cortex Tissue Lysate
    Lane 5 : Cerebellum Mouse Tissue Lysate
    Lane 6 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 53 kDa, 99 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    Junctional Adhesion Molecule 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The predicted molecular weight of Junctional Adhesion Molecule 1 is 32 kDa (SwissProt), however we expect to observe a banding pattern around 42 kDa. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125886 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

实验方案

文献

ab125886 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Mouse Tissue lysate - whole (intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
10 µg
Specification
intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 20 2015

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