Anti-JunB (phospho S259)抗体(ab30628)

概述

  • 产品名称Anti-JunB (phospho S259)抗体
    参阅全部 JunB 一抗
  • 描述
    兔多克隆抗体to JunB (phospho S259)
  • 特异性ab30628 recognises endogenous levels of JunB only when phosphorylated at Serine 259.
  • 经测试应用适用于: WB, ELISA, IHC-P, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human JunB around the phosphorylation site of Serine 259.

  • 阳性对照
    • Breast carcinoma, HeLa cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab30628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

Anti-JunB (phospho S259) antibody 图像

  • All lanes : Anti-JunB (phospho S259) antibody (ab30628) at 1/500 dilution

    Lane 1 : extracts from HeLa cells (5-30ug).
    Lane 2 : extracts from HeLa cells (5-30ug), preincubated with synthesized non phosphopeptide.
    Lane 3 : extracts from HeLa cells (5-30ug), preincubated with synthesized phosphopeptide (negative control).


    Predicted band size : 35 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 JunB (Phospho Ser259) Antibody (ab30628). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).

Anti-JunB (phospho S259) antibody (ab30628)参考文献

This product has been referenced in:
  • Suwei D  et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . Read more (PubMed: 26503334) »
  • Pérez-Benavente B  et al. GSK3-SCF(FBXW7) targets JunB for degradation in G2 to preserve chromatid cohesion before anaphase. Oncogene : (2012). Read more (PubMed: 22710716) »

See all 2 Publications for this product

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